The crystallization of lysozyme in the system of ionic liquid [BMIm][BF4]-water 书

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1、Cryst.Res.Technol.43,No.10,1062–1068(2008)/DOI10.1002/crat.200800040Thecrystallizationoflysozymeinthesystemofionicliquid[BMIm][BF4]-waterXinxinLi,XiaodongXu*,YuanyuanDan,JingFeng,LanGe,andMilinZhangKeyLaboratoryofSuperlightMaterialsandSurfaceTechnology,MinistryofEducation,HarbinEngineeringUniv

2、ersity,Harbin150001,P.R.ChinaReceived17March2008,revised21May2008,accepted23May2008Publishedonline25June2008Keywordscrystallization,singlecrystalgrowth,protein,lysozyme,ionicliquid.PACS87.15.Nn,81.10.Dn,87.14.Ee,81.10.-hLysozymecrystallizationwasconductedintheionicliquid(IL)1-butyl-3-methylimi

3、dizoliumtetrafluoroborate([BMIm][BF4])withdifferentbuffer/ILproportions.Itwasfoundthattheadditionof[BMIm][BF4]couldpromotethecrystallizationprocess,duringwhichmorelagersinglecrystalswithcontrollablemorphologiescouldbeobtainedduetothemanageablecrystalgrowthvelocity.Aprobableexplanationwaspropos

4、edbasedontheinfluenceoftheionicpolarizationandkineticsinthelysozymecrystallization.Moreover,thetransformincoordinationnumberandtherelativegrowthrateofdifferentcrystalfaceswerediscussed.©2008WILEY-VCHVerlagGmbH&Co.KGaA,Weinheim1IntroductionConsiderableeffortshavebeenpayedinthefieldofproteincrys

5、tallizationrecently.Anumberofproteinsinglecrystalshavebeenachieved[1-3],however,therearestillchallengestogetasinglecrystalforacertainprotein.Heretofore,therearenoconvincingtheoriesaboutpredictingcrystallizationconditionsforanewbiomacromoleculeorsomepersuasiveexplanationofwhytheproteinsinglecry

6、stalappearinginthatcondition,althoughFeher[4]andMcPherson[5]researcheditsprincipleandestablishedsomebasicwaystwentyyearsago.Themainobjectiveofproteincrystallizationistoacquireperfectcrystalandestablishits3Dstructure,whichhasspecialattractiontoproteomicsresearchers.Currently,biomacromoleculecry

7、stallizationisabottleneckinstructuralgenomics,whichalsoinfluencedtheproteinengineeringanddesigningmedicinepurposive[6-8].Commonchallengesincludemultipleortwincrystals,defectsincrystals,smallsizeofcrystalsandnon-reproducibilityofcrystall