A novel ionic liquid polymer material with high binding capacity for proteins

《A novel ionic liquid polymer material with high binding capacity for proteins》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、ViewOnline/JournalHomepage/TableofContentsforthisissueJournalofDynamicArticleLinksC

2、ngfeiPan,XiaoruiZhaiandShuoWang*Received15thSeptember2011,Accepted11thDecember2011DOI:10.1039/c2jm14577dInthisresearch,anewmacroporouspolymermaterialwithanexcellentadsorptioncapacityforproteinswassynthesizedinaqueousmediumbyusinganionicliquidpolymericmonomer,1-vinyl-

3、3-butylimidazoliumchloride(ViBuIm+Cl
).Itisthefirsttimeanionicliquidhasbeenchosenasafunctionalmonomertopreparepolymermaterialforproteinadsorption.Thepreparedionicliquidmaterialexhibitedstrongbindingabilityformanykindsofproteins,especiallyforlysozymewithamaximumbinding

4、capacityof755.1mgg
1undertheoptimumadsorptionconditions.Theionicliquidmonomerplayedanimportantroleintheproteinadsorptioncapacityofthematerial.Inaddition,therecognitionpropertyoftheionicliquidpolymermaterialcouldbeeasilytunedbychangingtheanionsoftheionicliquid.Themorp

5、hology,structure,compositionandthermalpropertiesoftheionicliquidmaterialwerefurthercharacterizedbyscanningelectronmicroscope(SEM),fouriertransforminfrared(FT-IR)spectroscopy,differentialscanningcalorimeter(DSC)andthermogravimetricanalyses(TGA).Easypreparationoftheion

6、icliquidpolymermaterialaswellashighabilitytoadsorbproteinsmakesthismaterialattractiveandbroadlyapplicableinbiomacromolecularseparation,biotechnology,assaysandsensors.Introductiongraftedsilicabeadswerepreparedthroughasurface-initiatedatomtransferradicalpolymerizationb

7、yKenichietal.4However,Duetothesignificanceofproteomicsformedicaldiagnosisandpreviousstudiesindicatedthatmostofthematerialsexhibitedtreatment,thelargescalestudyofproteinshasreceivedwide-DownloadedbyHenanNormalUniversityon15May2012aloweradsorptioncapacity(fromseveraltot

8、ensofmilligrams^cfspreadpublicattention.Proteinseparationisthereforeoneofthe5–8proteinspergrammaterials)andapoorbiocompatibility.Pu