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《Differential Expression and Activity of Tissue-nonspecificAlkaline Phosphatase (TNAP) in Rat Odontogenic Cells In Vivo组织非特异性的差异表达与活性 Alkaline Phosphatase（TNAP）在大鼠牙源性细胞中的作用》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、Volume47(12):1541–1552,1999TheJournalofHistochemistry&Cytochemistryhttp://www.jhc.orgARTICLEDifferentialExpressionandActivityofTissue-nonspecificAlkalinePhosphatase(TNAP)inRatOdontogenicCellsInVivoDominiqueHotton,NicoleMauro,FrédéricLézot,NadineForest,andArianeBerdalLaborat
2、oiredeBiologie–Odontologie,InstitutBiomédicaldesCordeliers(DH,NM,FL,NF,AB),andINSERMU458(DH),Paris,FranceSUMMARYAmongthefourexistingisoformsofalkalinephosphatase(AP),thepresentstudyisdevotedtotissue-nonspecificalkalinephosphatase(TNAP)inmineralizeddentaltissues.Northernblot
3、analysisandmeasurementsofphosphohydrolaseactivityonmicro-dissectedepitheliumandectomesenchyme,insituhybridization,andimmunolabelingonincisorsconfirmedthattheAPactiveinrodentteethisTNAP.WhereasthedevelopmentalpatternofTNAPmRNAandproteinandthepreviouslydescribedactivityweresi
4、milarinsupra-ameloblasticandmesenchymalcells,theydifferedinenamel-secretingcells,theameloblasts.AspreviouslyshownforotherproteinsinvolvedincalciumandphosphateKEYWORDShandlinginameloblasts,abiphasicpatternofsteady-stateTNAPmRNAlevelswasassoci-alkalinephosphataseatedwithaddit
5、ionalvariationsinameloblastTNAPproteinlevelsduringthecyclicmodula-bonetionprocess.AlthoughtheassociationofTNAPupregulationandtheinitialphaseofbio-dentinmineralizationappearedtobeabasicfeatureofallmineralizedtissues,ameloblasts(andenameltoalesserextent,odontoblasts)showedase
6、condselectivelyprominentupregulationofmineralizationTNAPmRNAproteinactivityduringterminalgrowthoflargeenamelcrystalsonly,i.e.,thevitaminDmaturationstage.ThisdifferentialexpressionactivityforTNAPinteethvsbonemayex-hypophosphatasiaplainthestrikingdentalphenotypevsbonerepor
7、tedinhypophosphatasia,ahereditaryinsituhybridizationdisorderrelatedtoTNAPmutation.(JHistochemCytochem47:1541–1552,1999)immunolocalizationAlkalinephosphatases(APs)areubiquitousin1994;Whyteetal.1995).Inrodents,thecDNAsen-manyspecies,frombacteriatohuman(Manesetal.codingforthep
8、lacental,embryonic,andintestinal1990).Theiroveralldistributionindicatesthattheseis
