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1、Volum~2~12,n~ml~r2,419~424FI~B$09/14M~y1991~,I~1F~d~rall~nofI~urop¢~nBioch~ml<~l$~¢i~II¢~O0t4Yl~l.#~l~$J.~t~ADONIS0014579~9100424QEndosomalassociationofaproteinphosphatasewithhighdephosphorylatingactivityagainstacoronavirusnucleocapsidproteinDevakiV.MohandasandSamue
2、lDalesC,vt'~bialollyGroup.DepartmentofMiirobiatogym~dh~mtunalogy,TheU~fft,¢r~lryafWesternOntario.LondomOntario.CanadaN6/f3C1Received18February1991:revisedversionreceived1:5March1991Ontheassumptionthatdepho~phoryiationofthen~urotropic.coronavirusJHM(JHMV)nuclcocap~ld
3、protein(NImaybeconnectedwithinitiationortheinfectiaascyclewe~arehedforarelevanthostenxymeactivity,Analysi~of~ubccllularfractionsfromL-2murin©flhrt~bla~ts,~parate¢lt~dualPercolldensityg,tadieats,revealedthepresenceofapho~phoprotei~phosphatase(PPPaseLco.~cdimcntinllwi
4、ththeendososomai/prelyso.s~im.Imaterial,whichpossesseshighactivityalainxtN,WithpurifiedpZPJNassubstratcitwasdemonstratedthatthisPPPase.dstinilaishablefromacidandtdkalin~phosph~tascs,actsoptimalb,atneutralpl'{inth©pres©nc¢ofMn]"followingtreatmentwithadetergent,Comple
5、teinhibitionwi:hokadaicacidat0.9.-4,$tIMbutnotatb,d0nMrelegatesthi~PPPasctontypeIproteinphosphatase.SimilarPPPaseactivilyforNwaspresentintheendosomefractionofatratRoc.1astrocytoma.olijlodendroc~ctecelllineandinhomc~lenatesofbrainandculturedOliltOdendrocytes.Oard~tas
6、ul/gestthatthephosphorylatcdNoftheinoculummaybemodifiedbytheendosomalPPPa~einhosl¢©11~,incladiniltho~cfromtheCNSsoa~tofactlitalethe.II.IMVinfectiousprocess.Phosphoproteinphosphatase;Endosome;Coronavirus;Nu¢leocapsidprotein1.INTRODUCTIONInthispaper,wepresentdataregar
7、dingpartialcharacterizationofaphosphoproteinphosphatase,Proteinphosphorylationisknowntobefundamentalassociatedwiththeendosomalfractionofhostcellsintheregulationofnumerouscellularprocesses[1].In(PPPase),whichishighlyactiveagainstthenucleocapsidtheeaseofvirusesdiffere
8、ntialstatesofphosphorylationproteinIN)ofJHMV.Amongthe3majorstructuralcanprofoundlymodulatecell-virusinteractionsandproteinsofJHMV,Nisphosp
