Overexpression of wheat ferritin gene TaFER-5B enhances tolerance to heat stress and other abiotic stresses associated with the ROS scavenging

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1、Zangetal.BMCPlantBiology(2017)17:14DOI10.1186/s12870-016-0958-2RESEARCHARTICLEOpenAccessOverexpressionofwheatferritingeneTaFER-5BenhancestolerancetoheatstressandotherabioticstressesassociatedwiththeROSscavenging††XinshanZang,XiaoliGeng,FeiWang,ZhenshanLiu,LiyuanZhang,YueZha

2、o,XuejunTian,ZhongfuNi,*YingyinYao,MingmingXin,ZhaorongHu,QixinSunandHuiruPengAbstractBackground:Theyieldofwheat(TriticumaestivumL.),animportantcrop,isadverselyaffectedbyheatstressinmanyregionsoftheworld.However,themolecularmechanismsunderlyingthermotolerancearelargelyunkno

3、wn.Results:Anovelferritingene,TaFER,wasidentifiedfromourpreviousheatstress-responsivetranscriptomeanalysisofaheat-tolerantwheatcultivar(TAM107).TaFERwasmappedtochromosome5BandnamedTaFER-5B.ExpressionpatternanalysisrevealedthatTaFER-5Bwasinducedbyheat,polyethyleneglycol(PEG)

4、,H2O2andFe-ethylenediaminedi(o-hydroxyphenylacetic)acid(Fe-EDDHA).ToconfirmthefunctionofTaFER-5Binwheat,TaFER-5BwastransformedintothewheatcultivarJimai5265(JM5265),andthetransgenicplantsexhibitedenhancedthermotolerance.Toexaminewhetherthefunctionofferritinfrommono-anddico-s

5、peciesisconserved,TaFER-5BwastransformedintoArabidopsis,andoverexpressionofTaFER-5Bfunctionallycomplementedtheheatstress-sensitivephenotypeofaferritin-lackingmutantofArabidopsis.Moreover,TaFER-5Bisessentialforprotectingcellsagainstheatstressassociatedwithprotectingcellsagai

6、nstROS.Inaddition,TaFER-5Boverexpressionalsoenhanceddrought,oxidativeandexcessironstresstoleranceassociatedwiththeROSscavenging.Finally,TaFER-5BtransgenicArabidopsisandwheatplantsexhibitedimprovedleafironcontent.Conclusions:OurresultssuggestthatTaFER-5Bplaysanimportantrolei

7、nenhancingtolerancetoheatstressandotherabioticstressesassociatedwiththeROSscavenging.Keywords:TaFER-5B,Heatstress,Abioticstress,Ferritin-lackingmutant,Wheat,ArabidopsisBackgroundincreasinglyimportant.Assessileorganisms,plantshaveMostoftheworld’swheatgrowingareasarefrequentl

8、yevolvedvariousresponsemechanismstoadapttoabioticsubjecttoheatstressduringthegrowi