Ectopic overexpression of wheat TaSrg6 gene confers water stress tolerance in Arabidopsis

Ectopic overexpression of wheat TaSrg6 gene confers water stress tolerance in Arabidopsis

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时间:2019-08-06

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1、PlantScience172(2007)10791086www.elsevier.com/locate/plantsciEctopicoverexpressionofwheatTaSrg6geneconferswaterstresstoleranceinArabidopsisa,b,c,da,b,c,da,b,c,dShaomingTong,ZhongfuNi,HuiruPeng,GuoqingDonga,b,c,d,QixinSuna,b,c,d,*aDepartmentofPlantGenetics&Breeding,StateKeyLabora

2、toryforAgrobiotechnology,ChinaAgriculturalUniversity,Beijing100094,ChinabKeyLaboratoryofCropHeterosisandUtilization,MinistryofEducation,ChinaAgriculturalUniversity,Beijing100094,ChinacKeyLaboratoryofCropGenomicsandGeneticImprovement,MinistryofAgriculture,ChinaAgriculturalUnivers

3、ity,Beijing100094,ChinadBeijingKeyLaboratoryofCropGeneticImprovement,ChinaAgriculturalUniversity,Beijing100094,ChinaReceived1February2007;receivedinrevisedform20March2007;accepted21March2007Availableonline31March2007AbstractTranscriptionalregulationplaysakeyroleinplantresponseto

4、abioticstresses.Studyinbarleyindicatedthatastress-responsivegene(Srg6),aspotentialtranscriptionalregulator,wasinducedbyvariousabioticstresses.However,functionalrolesofSrgproteinsinstresstoleranceareunknown.Inthisstudy,weisolatedawheathomologofbarleySrg6gene,anddesignatedTaSrg6.E

5、xpressionanalysisindicatedthatexpressionofTaSrg6geneisinducedbyvariousstresstreatments,includingPEG,salt,lowtemperatureandABA.ThefusionproteinGFP-TaSrg6wasconcentratedinthenucleuswhentransientlyexpressedinonionepidermalcells.OverexpressionofTaSrg6geneinArabidopsisconferredwaters

6、tresstoleranceintermsofsurvivalrate,CMSvalueandrelativewaterloss.WeconcludedthatTaSrg6proteinplayedanimportantroleintheenhancementofwaterstresstoleranceinplants.#2007ElsevierIrelandLtd.Allrightsreserved.Keywords:Arabidopsis;Waterstress;Overexpression;Stress-responsivegene;TaSrg6

7、;Wheat1.Introductioncascadethatcommenceswithperceptionandsubsequenttransductionofstresssignals,andthenleadingtotheactivationAbioticstressessuchasdrought,salinityorlowtemperatureofvariousstress-responsivegeneexpression[57].aremajorenvironmentalfactorsthathaveadverseeffectsonPervi

8、ousstudieshaveshownthatmanygenesareinducedplant

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