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1、lettersLossoffunctionoftheIAA-glucosehydrolasegeneTGW6enhancesricegrainweightandincreasesyieldKenIshimaru1,4,NaokiHirotsu1,4,YukaMadoka1,NaomiMurakami1,NaoHara1,HarukoOnodera1,TakayukiKashiwagi1,KazuhiroUjiie1,Bun-ichiShimizu2,AtsukoOnishi3,HisashiMiyagawa3&EtsukoKatoh
2、1Increasesintheyieldofrice,astaplecropformorethanInthisstudy,weclonedandfunctionallyanalyzedTGW6tofacili-halfoftheglobalpopulation,areimperativetosupportrapidtateimprovementsinyieldandtoultimatelysupportthebreedingofpopulationgrowth.Grainweightisamajordeterminingfactor
3、high-yieldcrops.ofyield.Here,wereportthecloningandfunctionalanalysisWecarriedouthigh-resolutionmappingwithhomozygousrecom-ofTHOUSAND-GRAINWEIGHT6(TGW6),agenefromthebinantplantsanddelimitedtheTGW6-containingregionto4.9kbIndianlandracericeKasalath.TGW6encodesanovelprotei
4、nbetweenthemarkersG214andG232.Inthisgenomicregion,therewithindole-3-aceticacid(IAA)-glucosehydrolaseactivity.wasonlyoneORF,Os06g0623700,predictedbytheRiceAnnotationInsinkorgans,theNipponbaretgw6alleleaffectsthetimingProject(RAP-DB;seeURLs)(Fig.1a).Thisputativegene,avia
5、bleofthetransitionfromthesyncytialtothecellularphasebycandidateforTGW6,hasonlyoneexon.TheKasalathallelecontainedcontrollingIAAsupplyandlimitingcellnumberandgrainsixnucleotidesubstitutionsanda1-bpdeletionatnucleotide313length.Mostnotably,lossoffunctionoftheKasalathallel
6、e(Fig.1b)comparedtotheNipponbareallele.The1-bpdeletioncausedenhancesgrainweightthroughpleiotropiceffectsonsourceaframeshiftandpreventedtheproductionofthematureprotein,organsandleadstosignificantyieldincreases.Ourfindingsowingtotheintroductionofaprematurestopcodon(Fig.1
7、bandsuggestthatTGW6maybeusefulforfurtherimprovementsinSupplementaryFig.1),whereasthesixnucleotidesubstitutionswereyieldcharacteristicsinmostcultivars.notexpectedtoaffecttheprotein.Therefore,theframeshiftmighthavegivenrisetofunctionallossoftheNipponbare-typeTGW6pro-Duri
8、ngthedomesticationofcrops,ourancestorsselectedsuperiortein.Inacomplementationtest,wetransformedanRNAinterfer-NatureAm