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1、MOLECULAR,CELLULAR,ANDDEVELOPMENTALBIOLOGYMolecularcloningandtissuedistributionofreducedfolatecarrierandeffectofdietaryfolatesupplementationontheexpressionofreducedfolatecarrierinlayinghens1M.Jing,*G.B.Tactacan,*J.C.Rodriguez-Lecompte,*A.Kroeker,*andJ.D.House
2、*†*DepartmentofAnimalScience,and†DepartmentofHumanNutritionalSciences,UniversityofManitoba,Winnipeg,Manitoba,R3T2N2,CanadaABSTRACTThereducedfolatecarrier(RFC)hastine,ovary,andtestis,aswellasothertissues.Real-beenpostulatedtobeamajorentityforfolatetrans-timePC
3、Ranalysisrevealedthatnosignificantdiffer-portactivityinhumansandothermammals.However,ences(P>0.05)duetodietwerefoundinthemRNAtherearelimitedreportsoftheimportanceofRFCinlevelsofRFCintheduodenumandcecum.However,anaviansystem.Inthecurrentstudy,therefore,thecomp
4、aredwiththebasaldiet,jejunalmRNAlevelsofmolecularcloningandtissuedistributionofRFC,asRFCweredecreased(P<0.05)inhensfedwiththewellastheeffectofdietaryfolatesupplementationon5-methyltetrahydrofolatediet,butthereductiondidtheexpressionofthistransporter,wereinves
5、tigatedinnotreachsignificance(P=0.077)inthehensfedthethechicken.ShaverWhitelayinghens(n=8perdiet)folicaciddiet.Overall,thecurrentstudydemonstratedreceived3wheat-baseddietscontainingthefollowing:thattheRFCcDNAcontainingthefullcodingregion1)nosupplementalfolate
6、,2)folicacid(10.00mg/kg),wassuccessfullyclonedfromtheduodenumoflayingor3)5-methyltetrahydrofolate(11.30mg/kg)for21d.hens.ThewidetissuedistributionofRFCtranscriptsThemRNAexpressionlevelswereanalyzedbyquantita-issuggestiveofanimportantroleofRFCintheprocesstiver
7、eal-timePCR.Theresultsshowedthattheclonedoffolatetransportinthechicken.Moreover,dietarypartialRFCcDNAcontainingthefullcodingregionfolatesupplementationcoulddownregulatethejejunalfromduodenumwas99%homologoustothereferencemRNAexpressionofRFC.Suchfindingswilllay
8、thegeneavailableinGenBank.AbroadexpressionprofilefoundationoffutureworkinvolvingtheRFCinavianofRFCtranscriptswasobserved,withRFCmRNAsystems,includinglayinghens.detectedinthebrain,liver,ki
