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1、ComparativeBiochemistryandPhysiology,PartA168(2014)25–39ContentslistsavailableatScienceDirectComparativeBiochemistryandPhysiology,PartAjournalhomepage:www.elsevier.com/locate/cbpaMechanistictargetofrapamycin(mTOR)signalinggenesindecapodcrustaceans:CloningandtissueexpressionofmTOR,Akt,Rh
2、eb,andp70S6kinaseinthegreencrab,Carcinusmaenas,andblackbacklandcrab,Gecarcinuslateralisaaba,⁎AliM.Abuhagr,KyleS.MacLea,ErnestS.Chang,DonaldL.MyklesaDepartmentofBiology,ColoradoStateUniversity,FortCollins,CO80523,USAbBodegaMarineLaboratory,UniversityofCalifornia-Davis,BodegaBay,CA94923,U
3、SAarticleinfoabstractArticlehistory:Mechanistictargetofrapamycin(mTOR)controlsglobaltranslationofmRNAintoproteinbyphosphorylatingReceived6August2013p70S6kinase(S6K)andeIF4E-bindingprotein-1.AktandRheb,aGTP-bindingprotein,regulatemTORproteinReceivedinrevisedform29October2013kinaseactivit
4、y.Moltingincrustaceansisregulatedbyecdysteroidssynthesizedbyapairofmoltingglands,orAccepted13November2013Y-organs(YOs),locatedinthecephalothorax.Duringpremolt,theYOshypertrophyandincreaseproductionofAvailableonline20November2013ecdysteroids.Rapamycin(1μM)inhibitedecdysteroidsecretioninC
5、arcinusmaenasandGecarcinuslateralisYOsinvitro,indicatingthatecdysteroidogenesisrequiresmTOR-dependentproteinsynthesis.TheeffectsofKeywords:TargetofrapamycinmoltingontheexpressionoffourkeymTORsignalinggenes(mTOR,Akt,Rheb,andS6K)intheYOwasinvesti-Rhebgated.PartialcDNAsencodinggreencrab(C.
6、maenas)mTOR(4031bp),Akt(855bp),andS6K(918bp)wereob-Akttainedfromexpressedsequencetags.Identity/similarityofthededucedaminoacidsequenceoftheC.maenasp70S6kinasecDNAstohumanorthologswere72%/81%forCm-mTOR,58%/73%forCm-Akt,and77%/88%forCm-S6K.MoltingmTOR,Akt,S6K,andelongationfactor2(EF2)inC.
7、maenasandblackbacklandcrab(G.lateralis)wereexpressedCrustaceainalltissuesexamined.ThetwospeciesdifferedintheeffectsofmoltingongeneexpressionintheYO.InY-organG.lateralis,Gl-mTOR,Gl-Akt,andGl-EF2mRNAlevelswereincreasedduringpremolt.Bycontrast,moltinghadEcdysteroidnoeffectontheexp
