分子克隆(molecular cloning) 第三版

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1、ColdSpringHarborLaboratoryPress-MolecularCloning-TableofContentsTableofContentsChapter1:PlasmidsandTheirUsefulnessinMolecularCloningChapter2:BacteriophageandItsVectorsChapter3:WorkingwithBacteriophageM13VectorsChapter4:WorkingwithHigh-CapacityVectors

2、Chapter5:GelElectrophoresisofDNAandPulsed-FieldAgaroseChapter6:PreparationandAnalysisofEukaryoticGenomicDNAChapter7:Extraction,Purification,andAnalysisofmRNAfromEukaryoticCellsChapter8:InVitroAmplificationofDNAbythePolymeraseChainReactionChapter9:Pre

3、parationofRadiolabeledDNAandRNAProbesChapter10:WorkingwithSyntheticOligonucleotideProbesChapter11:PreparationofcDNALibrariesandGeneIdentificationChapter12:DNASequencingChapter13:MutagenesisChapter14:ScreeningExpressionLibrariesChapter15:ExpressionofC

4、lonedGenesinEscherichiacoliChapter16:IntroducingClonedGenesintoCulturedMammalianCellsChapter17:AnalysisofGeneExpressioninCulturedMammalianCellsChapter18:ProteinInteractionTechnologiesProtocols

5、Bioinformatics

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17、SearchCopyright©2001byColdSpringHarborLaboratoryPress.Allrightsreserved.Nopartofthesepages,eithertextorimagemaybeusedforanypurposeotherthanpersonaluse.Therefore,reproductionmodifi

18、cation,storageinaretrievalsystemorretransmission,inanyformorbyanymeans,electronic,mechanical,orotherwise,forreasonsotherthanpersonaluse,isstrictlyprohibitedwithoutpriorwrittenpermission.http://www.molecularcloning.com/members/toc.jsp[2002-2-1816:10:2

19、3]ColdSpringHarborLaboratoryPress-MolecularCloning-Chapter1Chapter1PlasmidsandTheirUsefulnessinMolecularCloningProtocol1:PreparationofPlasmidDNAbyAlkalineLysiswithSDS:MinipreparationPlasmidDNAisisolatedfromsmall-scale(1-2ml)bacterialculturesbytreatme

20、ntwithalkaliandSDS.Protocol2:PreparationofPlasmidDNAbyAlkalineLysiswithSDS:MidipreparationPlasmidDNAisisolatedfromintermediate-scale(20-50ml)bacterialculturesbytreatmentwithalkaliandSDS.Protocol3:PreparationofPlasmidDNAbyAlkalineLysiswithSDS:Maxiprep