A method for electroporation during mammary gland development

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1、JMammaryGlandBiolNeoplasia(2013)18:233–237DOI10.1007/s10911-013-9292-6AMethodforElectroporationtoStudyGeneFunctioninMammaryGlandDevelopmentJong-MinLee&Eun-JungKim&Han-SungJungReceived:3February2013/Accepted:1May2013/Publishedonline:11May2013#SpringerScience+BusinessMediaNe

2、wYork2013AbstractTorevealthespecificfunctionsofvariousgenesEEmbryonicdayduringembryonicdevelopment,themanipulationofgenesusingPBSPhosphatebufferedsalinetechniquessuchaselectroporationisoffundamentalimportanceEGFPEnhancedGreenFluorescentProteinforprovidingdirectevidenceconc

3、erningfunctionordown-streamactivationofsignalingnetworks.InvitroembryocultureandelectroporationareusefultechniquestointroduceforeignIntroductiongenes,fordevelopmentalbiologystudies.Amongthevariousmammalianculturetechniques,TrowellcultureissuitableforThemouseembryoiscurrent

4、lythemostenlighteningexper-studiesofembryonicmammaryglanddevelopmentbecauseofimentalmodelsystemforstudyingvertebratedevelopment.itsstabilityandeaseofuseinconjunctionwithelectroporationSincethe1970s,cultureofwholerodentembryoshasrevealedtechniqueapplication.Themanipulationo

5、fgeneexpressionthecausesofdevelopmentaleventssuchasmorphogenesis,usingelectroporationisausefultechniqueforthefunctionalcellproliferationandcellmigration[1,2].Moreover,methodsanalysisofaparticulargene.Inthisprotocol,fullstepsforforgrowingwholemouseembryosinvitrohavebeensign

6、if-electroporationandinvitroembryoculturehavebeendescribedicantlyimproved[2].foruseinembryonicmammaryglanddevelopmentresearch.Variousmouseembryoculturemethodshavebeendevel-opedinthelast30years[3].Amongtheculturemethods,KeywordsElectroporation.Invitroembryoculture.Trowellcu

7、ltureisthemostfunctionalwaytoobservedetailedGenetransfer.Mouse.Mammaryglandmorphogenesisnotonlyinorgansandintissueculturebutalsobywholeembryoculture.Trowellcultureisanadequatecul-Abbreviationsturesystem,especiallyforstudiesofembryonicmammaryMRMammaryrudimentsglanddevelopme

8、nt,becauseofitssimpleprocessesformanip-ICRInstituteofCancerResearchulatinggenesandforconf