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1、AnalyticaChimicaActa905(2016)115e125ContentslistsavailableatScienceDirectAnalyticaChimicaActajournalhomepage:www.elsevier.com/locate/acaDevelopmentofamethodforenhancingmetabolomicscoverageofhumansweatbygaschromatographyemassspectrometryinhighresolutionmodea,b,ca,b,c,**a,b,cM.M.Delgado
2、-Povedano,M.Calderon-Santiago,F.Priego-Capote,a,b,c,*M.D.LuquedeCastroaDepartmentofAnalyticalChemistry,AnnexMarieCurieBuilding,CampusofRabanales,UniversityofCordoba,Cordoba,SpainbceiA3AgroalimentaryExcellenceCampus,UniversityofCordoba,Cordoba,SpaincMaimonidesInstituteofBiomedical
3、Research(IMIBIC),ReinaSofíaUniversityHospital,Cordoba,SpainhighlightsgraphicalabstractMethyoxiaminationandsilylationafterdeproteinizationforasnapshotofsweatmetabolome.GCeTOF/MS,anexcellentstrategytoanalyzesweatintermsofmetab-olomecoverage.ComparisonoftheGCeTOF/MSstudywithprevious
4、studiesusingNMRorLCeMS.Sixty-sixcompoundsweretenta-tivelyidentifiedbytheobtainedMSinformation.articleinfoabstractArticlehistory:Sweathasrecentlygainedpopularityasclinicalsampleinmetabolomicsanalysisasitisanon-invasiveReceived15September2015biofluidthecompositionofwhichcouldbemodifiedbyc
5、ertainpathologies,asisthecasewithcysticReceivedinrevisedformfibrosisthatincreaseschloridelevelsinsweat.However,thewholecompositionofsweatisstillun-3November2015knownandthereisalackofanalyticalstrategiesforsweatanalysis.TheaimofthepresentstudywastoAccepted26November2015developandvalidat
6、eamethodformetabolomicanalysisofhumansweatbygaschromatographyetimeAvailableonline17December2015offlight/massspectrometry(GCeTOF/MS)inhighresolutionmode.Thus,differentsamplepreparationstrategieswerecomparedtochecktheireffectontheprofileofsweatmetabolites.Sixty-sixcompoundsKeywords:werete
7、ntativelyidentifiedbytheobtainedMSinformation.Aminoacids,dicarboxylicacidsandotherMetabolomicsGlobalprofilinginterestingmetabolitessuchasmyo-inositolandurocanicacidwereidentified.Amongthetestedpro-Humansweattocols,methyoxiaminationplussilylationafterdeproteinizationwasthemostsuitedoption
8、toobt
