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1、中华神经外科疾病研究杂志(ChinJNeurosurgDisRes)2008;7(2)·149·文章编号:1671-2897(2008)07-149-05·论著·高压氧预处理对大鼠脊髓损伤后GFAP和巢蛋白表达的影响3卢培刚冯华王宪荣卢佳友胡胜利夏勇智储卫华宫国旗(第三军医大学西南医院神经中心,重庆400038)摘要目的探讨高压氧预处理对成年大鼠脊髓损伤后不同时期巢蛋白(nestin)和胶质纤维酸性蛋白(GFAP)表达变化的影响。方法成年SD大鼠55只,雌性,体重250～300g。随机分为高压氧预处理组、正常损伤组各25只(n=5),对照组5
2、只。采用改良Allen's法制作模型,分别于伤后1d、5d、7d、10d和14d取材,免疫组化染色及图像分析检测组织中GFAP和巢蛋白的表达。结果对照组显示除脊髓中央管周围可见到少量巢蛋白表达外,其它部位几乎不表达;GFAP在脊髓各个部位均有表达;正常脊髓损伤组:1d损伤区域巢蛋白和GFAP表达增加;5d表达显著增加;7d达高峰;10～14d逐渐下调,与对照组差异明显;高压氧预处理组:各时间段均与正常损伤组有明显差异(P<0.05)。结论高压氧预处理可诱导巢蛋白高表达和星形胶质细胞增生,对中枢神经系统损伤起到保护作用。关键词高压氧;预处理;脊
3、髓损伤;胶质纤维酸性蛋白;巢蛋白中国图书资料分类号R651.2文献标识码AEffectsofpreconditioningwithhyperbaricoxygenonexpressionofGFAPandnestinafterspinalcordinjuryinratsLUPeigang,FENGHua,WANGXianrong,LUJiayou,HUShengli,XIAYongzhi,CHUWeihua,GONGGuoqiDepartmentofNeurosurgery,SouthwestHospital,ThirdMilitaryMed
4、icalUniversity,Chongqing400038,ChinaAbstractObjectiveTostudytheeffectsofpreconditioningwithhyperbaricoxygen(HBO)onexpressionofglialfibrilaryacidicprotein(GFAP)andnestinafterspinalcordinjuryinratsatdifferenttime.MethodsFifty2fiveSprague2Dawleyadultrats,weighingfrom250to300g,
5、wererandomlydividedinto3groups,hyperbaricoxygenpreconditionalgroup(25,n=5),normalinjurygroup(25,n=5)andcontrolgroup(5,n=5).Intheexperimentalgroups,theratspinalcordinjurymodelswereestablishedbythewayofAllen'smethodandtheexpressionandproliferationofnestinandGFAPatdifferenttim
6、e(1d,5d,7d,10d,14d)wereobservedwithimmunohistochemicalstainingandtheanalyticalsystemofphotographs.ResultsInthenormalcontrolgroup,theexpressionofnestinwashardlyseenexceptependymalcellsofcentralcanal,andthelowexpressionofGFAPpresentedineveryparts.Intheexperimentalgroups,thene
7、stinandGFAPexpressionsincreasedobviouslyintheinjuredsitesandadjacentsites1dto5dafterinjury,reachedthepeakafter7dandfollowedbygradualdecreasefrom10dto14d.TherewerestatisticallysignificantdifferencesinthenestinandGFAPexpressionsbetweencontrolgroupandnormalinjurygroup,andbetwe
8、ennormalinjuryandhyperbaricoxygenpreconditionalgroup.ConclusionTheaboveresultssugg
