甲强龙预处理对大鼠脊髓损伤后claudin-5表达的影响.pdf

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1、中国神经精神疾病杂志2014年第40卷第12期741论著·甲强龙预处理对大鼠脊髓损伤后claudin一5表达的影响☆崔敏周昌龙贺学农夏小辉张光伟【摘要】目的探讨甲强龙(118，17旺，21一三羟基一60L一甲基孕甾一1,4一二烯一3，20二酮一21一琥珀酸钠)对大鼠脊髓损伤(spinalcordinjury，SCI)后血一脊髓屏障(bloodspinalcordbarrier，BSCB)通透性及紧密连接蛋白claudin一5表达的影响。方法参照改良Allen’S法建立大鼠脊髓损伤模型。分为假手术组(n=5

2、0)、脊髓损伤组(n=-50)、甲强龙预处理组(n=50)。建模后不同时间点(12h、ld、3d、5d、7d)各组随机取5只大鼠检测BSCB通透性，取胸段脊髓损伤区组织经RT—PCR、Westernblot方法检测claudin一5表达。结果本实验制模成功率84．O％；脊髓损伤组各时间点EB含量较假手术组显著增高(F=27．732，P均<0．05)。甲强龙预处理组各时间点EB含量较脊髓损伤组显著降低(F=48．149，P均<0．05)；脊髓损伤组各时间点claudin一5mRNA表达较假手术组显著降低(1

3、2．248，P均<0．05)。甲强龙预处理组各时间点claudin一5mRNA表达较脊髓损伤组显著升高(F=15．316，P均<0．O5)；脊髓损伤组各时间点claudin一5表达较假手术组显著降低(F=18．108，P均

4、1【文献标识码】ATheefectsofmethylprednisolonepretreatmentontheclaudin-5expressionafterspinalcordinjuryinrats．CU／Min，ZHOUChanglong,HEXuenong,XIAXiaohui，ZHANGGuangwei．Departmentofneurosurgery,theyongchuanaffiliatedhospital,Chongqingmedicaluniversity,439xuanhuaroad

5、，yongchuanDistrict，Chongqing402160．Tel：023—85382926．[Abstract]ObjectiveTostudytheeffectsofmethylpIlednisoloneonthepermeabilityofblood—spinalcordbarrier(BSCB)andclaudin一5expressionafterspinalcordinjuryinrats．MethodsTheratmodelofspinalcordinjurywasestab—lis

6、hedusingmodifiedAlienmethod．SDratswererandomlydividedintosham-operatedgroup，spinalcordinjurygroupandmethylprednisolonepretreatmentgroup．ThepermeabilityofBSCBandexpressionofclaudin一5wereassessedat12h，1，3，5，and7daftertheonsetofspinalcordinjuryveanimalsperea

7、chtimepoint)．RT-PCRandWesternblotwereusedtodetecttheexpressionofclaudin一5．ResultsTheSuccessrateofthemodelwas84．O％．EBcontentwassig—nificantlyhigherinspinalcordinjurygroupthaninsham-operatedgroupateachtimepointfFvalue27．732，P

8、prednisol0nepretreatmentgroupthaninspinalcordinjurygroup(Fvalue48．149，P<0．05)．ThemRNAexpressionofclaudin一5waslowerinspinalcordinjurygroupthansham—operatedgroupateachtimepointfFvalue12．248，P<0．05)．ThemRNAexpressionof