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1、66中国脊柱脊髓杂志2006年第16卷增刊ChineseJournalofSpineandSpinalCord,2006,Vol.16,Suppl甲基强的松龙对大鼠脊髓损伤后GFAP,neurocan和phosphacan表达的影响焦鹰，朱悦(中国医科大学附属第一医院骨科110001辽宁省沈阳市)I摘要】目的:观察甲基强的松龙(MP)对大鼠脊髓损伤(SCI)后GFAP,neurocan和phosphacan表达的作用。方法:36只成年Wistar大鼠随机分为3组，其中正常对照组4只，损伤组和治疗组各16只。正
2、常对照组大鼠不损伤脊髓，治疗组大鼠采用局部压迫法损伤T9脊髓，损伤后立即尾静脉推注MP30mg/kg，随后24h内每间隔6h推注MP(30mg/kg)一次，共给药5次。损伤组以同样方法损伤脊髓并推注等量生理盐水。分别于伤后3d,7d及14d切取损伤处脊髓标本，行RT-PCR及免疫组化检测GFAP,neurocan和phosphacanmRNA相对含量和蛋白表达。结果:大鼠SCI后GFAP,neurocan和phosphacanmRNA相对含量增加。伤后3d,7d,14d,MP治疗组比损伤组GFAPmRNA含量
3、均明显减少(P<0.01);伤后3d,7d治疗组neurocan和phosphacanmRNA含量减少(P<0.01);至伤后14d时含量改变不明显(P50.05)。结论:SCI后MP能抑制大鼠GFAP,neurocan和phosphacan表达。【关键词】脊髓损伤;甲基强的松龙;胶质纤维酸性蛋白;硫酸软骨素蛋自多糖中图分类号:R683.2,R977.1文献标识码:A文章编号:1004-406X(2006卜Suppl-0066-04Theeffectofmethylprednisoloneontheexpre
4、ssionofglialfibrillaryacidicprotein,neurocanandphosphacanafterspinalcordinjuryinrats/JIAOYing,ZHUYue//ChineseJournalofSpineandSpinalCord,2006,16(Suppl):66-69[Abstract]Objective:ToinvestigatethedownregulatedeffectofMethylprednisolone(MP)ontheexpressionofglia
5、lfibrillaryacidicprotein(GFAP),neurocanandphosphacaninspinalcordinjury(SCI)rats.Method:36adultwistarratswererandomlyassignedtothreegroupswith3incontro1,16inSCIandSCI+MPgrouprespec-tively.Nolesionstothespinalcordwereproducedinanimalsinthenormalgroup.SCIanima
6、lmodelwasin-ducedbytemporaryplacementofaweight(10g)at叨for30seconds.AnimalswithSCIweretreatedwithMP(30mg/kgsc)oranequalvolumeofsaline.Immediatelyafterthespinalcordwasinjured,abolusinjectionofMPsodiumsuccinate(30mg/kg)wasadministeredviatailvein,followedbyfour
7、subsequentadministrationsofMP(30mg/kg)at6hrintervals.Immunohistochemistryandsemiquantitativereversetranscription-PCRwereusedtoexaminetheexpressionofGFAP,neurocanandphosphacanat3d,7dand14dpostinjury.Result:TheexpressionofGFAPandneurocan,phosphacanmRNAincreas
8、edafterSCI.TheupregulationofGFAPmRNAwaslimitedinSCI+MPgroupat3d,7dand14dpostinjury(P<0.01),theupregulationofneurocanandphos-phacanmRNAwaslimitedinSCI+MPgroup3d,7dpostinjury(P<0.01),withnodifferenceinne
