5种中药体外抗鼻咽癌作用筛选与石上柏提取物诱导鼻咽癌细胞凋亡机制的分析

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1、硕士研究生学位论文5种中药体外抗鼻咽癌作用筛选及石上柏提取物诱导鼻咽癌细胞凋亡机制的研究FIVEKn奶S0FTRADITIONALCHn呵ESEMEDICINEANTI．NPCINVITROANDSDHEXTRACTINDUCEDAPOPTOSISOFNPCCELLSABSTRACTBackgroundand0bjeetive：NasopharyngealcarcinomaOqpc)istom-mortinsoutheastofChina，andthe5-year-survivalrateisonly50％～70％afterradiotherapyandchemotherapy,radio

2、resistance，chemoresistanceandseverecomplicationslowertheeffectofcombiningtreatmentfornasopharyngealCar—cinoma．Thesearchformoreeffectiveandlesstoxicsideeffectsofanti．NPCdrugsandimproveefficacyofclinicaltreatmentisallurgentneedtoaddresstheproblem．TraditionalChinesemedicine(TCM)isthatpeopleconstantly

3、sumup，selectionderivedatlifeanddisease，proventobeeffective．Westudythesensi-tivityofhumanNPCcellstofivekindsofcommonTCMinGuangxithroughNPCChemosensitivityAssayinvitro，andanti-NPCmechanismsofthebestonethroughCellbiologyandmolecularbiology．Method：ExtractionoffivekindsofTCMingredientsrespectively,incl

4、ud-ingScutellariabarbataD．Don(SB)，Hedyotisdiffusa(HD)，HerbaAgrimo—niae(HA)，Nitidine(ND)，SelaginelladoederleiniiHieron(SDH)．CNE1andCNE2NPCcellweretreatedwiththesefiveLiquidExtractsanddrugcontainingserumatdifferentconcentration，serumpharmacologyandMethylThiazolylTetrazolium(MTT)assayinvitrowereusedt

5、omeasuretheinhibitionrateofdrugsongrowthofNPCcells．TW03，HONE1NPCcellsandHFL．Ihuman5硕士研究生学位论文5种中药体外抗鼻咽癌作用筛选及石上柏提取物诱导鼻咽癌细胞凋亡机制的研究embryoniclungfibroblastcellweretreatedwithSDHatdifferentconcentration，MTTassaywasusedtoobservetheeffectofSDHongrowthofthreecelllines；Phasecontrastmicroscopewasusedtoobserv

6、ethecellsmorphologychanged．ThechangeoncellcyclewasanalyzedbyPropidiumIodide(PI)stainingandflowcytometry(FCM)．AnnexinV—FITCandPIstainingFCMmethodwasusedtoobservetheapoptosisoftheNPCcells．TheexpressionchangesofBaxandBcl一2inNPCcellsinducedbySDHweredetectedbyImmunohistochemistry．Results：SDHExtractandS

7、DHcontainingserumsignificantlyinhibitedthegrowthandProliferationofCNE1，CNE2cellsintimeanddosedependentmanner．AcertainconcentrationofNDExtractandNDcontainingserumcouldinhibitthegrowthofCNE1，CNE2cells．Highconcentra