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1、ABAC-BasedPhysicalMapofZhikongScallop(ChlamysfarreriJonesetPreston)11,21,21,2111,2XiaojunZhang,CuiZhao,ChaoHuang,HuDuan,PinHuan,ChengzhangLiu,XiuyingZhang,3131YangZhang,FuhuaLi,Hong-BinZhang,JianhaiXiang*1KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyof
2、Sciences,Qingdao,China,2GraduateUniversityoftheChineseAcademyofSciences,Beijing,China,3DepartmentofSoilandCropSciences,TexasA&MUniversity,CollegeStation,Texas,UnitedStatesofAmericaAbstractZhikongscallop(Chlamysfarreri)isoneofthemosteconomicallyimportantaquaculturespeciesinChina.Physi
3、calmapsarecrucialtoolsforgenomesequencing,genemappingandcloning,geneticimprovementandselectivebreeding.Inthisstudy,wehavedevelopedagenome-wide,BAC-basedphysicalmapforthespecies.Atotalof81,408clonesfromtwoBAClibrariesofthescallopwerefingerprintedusinganABI3130xlGeneticAnalyzerandafing
4、erprintingkitdevelopedinourlaboratory.Afterdataprocessing,63,641(,5.86genomecoverage)fingerprintswerevalidatedandusedinthephysicalmapassembly.Atotalof3,696contigswereassembledforthephysicalmap.Eachcontigcontainedanaverageof10.0clones,withanaveragephysicalsizeof490kb.Thecombinedtotalp
5、hysicalsizeofallcontigswas1.81Gb,equivalenttoapproximately1.5foldofthescallophaploidgenome.Atotalof10,587BACendsequences(BESs)and167markerswereintegratedintothephysicalmap.Weevaluatedthephysicalmapbyovergohybridization,BAC-FISH(fluorescenceinsituhybridization),contigBACpoolscreeninga
6、ndsourceBAClibraryscreening.Theresultshaveprovidedevidenceofthehighreliabilityofthecontigphysicalmap.Thisisthefirstphysicalmapinmollusc;therefore,itprovidesanimportantplatformforadvancedresearchofgenomicsandgenetics,andmappingofgenesandQTLofeconomicalimportance,thusfacilitatingthegen
7、eticimprovementandselectivebreedingofthescallopandothermarinemolluscs.Citation:ZhangX,ZhaoC,HuangC,DuanH,HuanP,etal.(2011)ABAC-BasedPhysicalMapofZhikongScallop(ChlamysfarreriJonesetPreston).PLoSONE6(11):e27612.doi:10.1371/journal.pone.0027612Editor:TongmingYin,NanjingForestryUniversi
8、ty,ChinaRece
