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1、中国组织工程研究与临床康复第14卷第47期2010–11–19出版JournalofClinicalRehabilitativeTissueEngineeringResearchNovember19,2010Vol.14,No.47人脐静脉脱细胞基质的制备★12222王海江，范应中，李泸平，张震，张大Preparationofextracellularmatrixofhumanumbilicalveins12222WangHai-jiang,FanYing-zhong,LiLu-ping,ZhangZhen,ZhangDaAbstract1BACKGROUND
2、:Preparationofextracellularmatrix(ECM)aimstoremovethecellularcomponentsintissues,thereisnoDepartmentofSurgery,TheThirduniformstandardforthecurrentacellularmethods,butthepreparedECMshouldbeprovedtobewithoutresidualcells.People’sHospitalofOBJECTIVE:TopreparehumanumbilicalveinECMbyusin
3、ghypotonicandhypertonicsolutions,detergentandproteinaseinaShenzhen,Shenzhenmultistepprocess,mixedsolutionofTritonX-100andammoniawater,andtoexploretheidealmethodofpreparinghuman518020,GuangdongProvince,China;umbilicalveinECM.2DepartmentofMETHODS:ECMofhumanumbilicalveinswaspreparedbyu
4、seofhypotonicandhypertonicsolutions,detergentandPediatricSurgery,proteinaseinamultistepprocessandsolutionofTritonX-100andNH3·H2O.Thephysicalpropertiesandhematoxylin-eosinTheFirstAffiliatedHospitalofstainingofhumanumbilicalveinbeforeandafteracellularmatrixwereobserved,theamountofresi
5、dualcelldebrisin50imagesZhengzhouat400magnificationwasmeasured.University,RESULTSANDCONCLUSION:Theacellularumbilicalveinsexhibitedtranslucentporcelain-whitejelly-liketubularstructure.Zhengzhou450052,HenanThecellcomponentsofthehumanumbilicalveinsresultingfromhypotonicandhypertonicsol
6、utions,detergentandproteinaseProvince,Chinawerealmostremoved,ECMmaintainedanintegrity,andfibrousstructureswerepreserved.ECMresultingfromsolutionofTriton-X100andNH3·H2Ohaddifferentquantitiesofremainingcellularelements.ThereweresignificantdifferencesbetweentheWangHai-jiang★,twogroups(
7、P<0.05).Hypotonicandhypertonicsolutions,detergentandproteinaseareidealmethodstoprepareECMfromMaster,Attendingphysician,humanumbilicalveins.DepartmentofSurgery,TheThirdWangHJ,FanYZ,LiLP,ZhangZ,ZhangD.Preparationofextracellularmatrixofhumanumbilicalveins.ZhongguoZuzhiPeople’sHospitalo
8、fShenzhen,ShenzhenG
