资源描述：
《Generation and characterization of a functional Nanobody against the vascular endothelial growth factor receptor-2; angiogenesis cell receptor》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、MolecularImmunology50(2012)35–41ContentslistsavailableatSciVerseScienceDirectMolecularImmunologyjournalhomepage:www.elsevier.com/locate/molimmGenerationandcharacterizationofafunctionalNanobodyagainstthevascularendothelialgrowthfactorreceptor-2;angiogenesiscellreceptorMahdiaa,∗aabBehdani,SirousZeinal
2、i,HosseinKhanahmad,MortezaKarimipour,NaderAsadzadeh,Keyhancad,eaAzadmanesh,AlirezaKhabiri,SteveSchoonooghe,MahdiHabibiAnbouhi,GholamrezaHassanzadeh-Ghassabehd,ed,e,SergeMuyldermansaDepartmentofMolecularMedicine,PasteurInstituteofIran,Tehran,IranbDepartmentofAnimalBreedingandGenetics,AnimalScienceRes
3、earchInstituteofIRAN(ASRI),IrancDepartmentofVirology,PasteurInstituteofIran,Tehran,IrandLaboratoryofCellularandMolecularImmunology,VrijeUniversiteitBrussel,Brussels,BelgiumeDepartmentofStructuralBiology,NSF,VIB,Brussels,BelgiumarticleinfoabstractArticlehistory:Vascularendothelialgrowthfactorreceptor
4、-2(VEGFR2)isanimportanttumor-associatedreceptorandReceived5November2011blockadeoftheVEGFreceptorsignalingcanleadtotheinhibitionofneovascularizationandtumorReceivedinrevisedformmetastasis.Nanobodiesarethesmallestintactantigenbindingfragmentsderivedfromheavychain-onlyNovember201125antibodiesoccurringi
5、ncamelids.Here,wedescribetheidentificationofaVEGFR2-specificNanobody,Accepted29November2011namedAvailableonline29December20113VGR19,fromdromedariesimmunizedwithacelllineexpressinghighlevelsofVEGFR2.WedemonstratebyFACS,that3VGR19NanobodyspecificallybindsVEGFR2onthesurfaceof293KDRandHUVECscells.Furthermo
6、re,the3VGR19Nanobodypotentlyinhibitsformationofcapillary-likestruc-Keywords:tures.VEGFR2ThesedatashowthepotentialofNanobodiesfortheblockadeofVEGFR2signalingandprovideabasisNanobodyforthedevelopmentofnovelcancertherapeutics.Angiogenesis©2011ElsevierLtd.Allrightsreserved.1.Introductionreceptorsignalin
7、gmayleadtotheinhibitionofneovascularizationandtumormetastasis.Angiogenesisplaysanimportantroleinthegrowth,invasionSerumofcamelidaecontainsanimportantfractionoffunc-andmetastasisofcancer.Blockadeofangi
