资源描述：
《没食子酰氧化芍药苷在皮肤角质化细胞中的抗氧化应激作用》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、OriginalArticleBiomolTher21(5),349-357(2013)6'-O-GalloylpaeoniflorinProtectsHumanKeratinocytesAgainstOxidativeStress-InducedCellDamageChengWenYao,MeiJingPiao,KiCheonKim,JianZheng,JiWonChaandJinWonHyun*SchoolofMedicineandInstituteforNuclearScienceandTechnology,JejuNationalUniversit
2、y,Jeju690-756,RepublicofKoreaAbstract6'-O-galloylpaeoniflorin(GPF)isagalloylatedderivateofpaeoniflorinandakeychemicalconstituentofthepeonyroot,aperen-nialfloweringplantthatiswidelyusedasanherbalmedicineinEastAsia.ThisstudyisthefirstinvestigationofthecytoprotectiveeffectsofGPFagainsthy
3、drogenperoxide(HO)-inducedcellinjuryanddeathinhumanHaCaTkeratinocytes.GPFdemonstrated22asignificantscavengingcapacityagainstthe1,1-diphenyl-2-picrylhydrazyl(DPPH)freeradical,HO-generatedintracellularre-22activeoxygenspecies(ROS),thesuperoxideanionradical(O-),andthehydroxylradical(•
4、OH).GPFalsosafeguardedHaCaT2keratinocytesagainstHO-provokedapoptoticcelldeathandattenuatedoxidativemacromoleculardamagetoDNA,lipids,and22proteins.ThecompoundexerteditscytoprotectiveactionsinkeratinocytesatleastinpartbydecreasingthenumberofDNAstrandbreaks,thelevelsof8-isoprostane(a
5、stableend-productoflipidperoxidation),andtheformationofcarbonylatedproteinspecies.Takentogether,theseresultsindicatethatGPFmaybedevelopedasacytoprotectoragainstROS-mediatedoxidativestress.KeyWords:6'-O-Galloylpaeoniflorin,HaCaTkeratinocyte,Hydrogenperoxide,Oxidativestress,Reactiveo
6、xygenspeciesINTRODUCTIONoticcells(Farber,1994).Furthermore,HOforms•OHand22ahydroxylanion(OH-)inthepresenceoftheferrousionandReactiveoxygenspecies(ROS)(e.g.,thesuperoxideanionotherheavymetalsasaconsequenceoftheFentonreactionradical(O-),singletoxygen(1O),hydrogenperoxide(HO),(Pignat
7、elloetal.,2006).2222andthehighlyreactivehydroxylradical(•OH))arederivedUndernormalconditions,thecellsofaerobicorganismsfromthemetabolismofmolecularoxygen(Halliwell,1999).IncontainsafelevelsofROS,whicharecounterbalancedbybio-mammaliancells,potentialenzymaticsourcesofROSincludechemi
8、calantioxidants.TheseROSplayanumb
