资源描述：
《可视膜片钳法记录初级传入纤维介导的脊髓背角神经元突触后电流》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、550ActaPhysiologicaSinica,August25,2004,56(4):550-557http://www.actaps.com.cnExperimentalTechniqueVisuallyguidedpatch-clamprecordingofspinaldorsalhornneuron’spostsynapticcurrentevokedbyprimaryafferentfiberWANYe-Hong,WANGYu-Ying,DAIFei,HUSan-Jue*InstituteofNeuroscience,TheFourthMilitaryMedicalU
2、niversity,Xi’an,Shaanxi710033,ChinaAbstract:Theauthorsdescribeheretheproceduresforusingthegelatinhalf-embeddingmethodtoobtainthinspinalcordsliceswithattacheddorsalrootsandperformingvisuallyguidedwhole-cellpatch-clamprecordingofpostsynapticcurrentsevokedbyprimaryafferentfibersinratspinaldorsalh
3、orn.Asegmentofspinalcordwithattacheddorsalrootswaspreparedandhalf-embeddedinanagarblockwith20%(w/v)gelatin.Thinspinalcordsliceswithattacheddorsalrootswereobtainedwithavibratomeandwhole-cellpatch-clampconfigurationwasestablishedundertheinfraredobservation.Attheholdingpotentialof70mV,spontaneous
4、excitatorypostsynapticcurrents(EPSCs)anddorsalrootstimulation-evokedEPSCswererecordedasinwardcurrents.Accordingtotheconductionvelocityofafferentfibersandstimulusthreshold,evokedEPSCsthataremediatedbyA-likeorC-likefibersweredistinguished.Attheholdingpotentialof0mV,spontaneousinhibitorypostsynap
5、ticcurrents(IPSCs)anddorsalrootstimulation-evokedIPSCswererecordedasoutwardcurrents.Using5µmol/Lstrychnineor20µmol/Lbicuculline,GABAergicorglycinergicevokedIPSCscouldbeisolated.Usingvisualpatch-clampmethodsynaptictransmissioncanbeaccuratelyassessedbymeasuringpostsynapticcurrentsofthedorsalhorn
6、neurons.Moreimportantly,withtheaidofinfraredobservation,theincidenceoffailuretoestablishaclampconfigurationcanbegreatlyreducedanditbecomeseasiertomakerecordingsfromtheneuronsindeepdorsalhornlaminae.Thus,thepresentresearchapproachaneffectiveapproachtostudythemodulationofprimaryafferentsynaptict
7、ransmission.Keywords:patch-clamptechnique;excitatorypostsynapticcurrent(EPSC);inhibitorypostsynapticcurrents(IPSC);primaryafferentsynapse;spine;dorsalhorn;rat*,710033:,,20%,,70mV,,AC0mV,5µmol/L20µmol/Lγ-,,,,:;;;;;;:Q424;R338Received2003
