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1、Effectsoffishmealqualityandfishmealsubstitutionbyanimalproteinblendongrowthperformance，GH/IGF-IaxisandfleshqualityofJapaneseseabass(Lateolabraxjaponicus)HU.L.,XUEM*.WANGL,WUX,GEH,ZHENGYNationalAqua-feedSafetyAssessmentStation,FeedResearchInstitute,CAASISFNF2010,Qingdao,CHINANational
2、Aqua-feedSafetyAssessmentStation,FRI,CAAS,Beijing,P.R.CHINAJapaneseseabass(Lateolabraxjaponicus)CarnivorousWidelyculturedinbothsaltyandfreshwaterHighestproductionamongmarinespeciesinChina.4BackgroundandJustificationFishmealsubstitution:Supply,price,sustainabilityAnimalproteinblend:A
3、minoacidcomplementation,costsavings,sustainability,PalatabilityforCarnivorousfishspecies(vs.plantsources)DIAA:MoreprecisethandeterminedAAlevel,lowercrudeproteinformulation,reducingwaste,LiteraturesreviewManyfactorssuchasculturingsystem,husbandry,fishspecieswouldaffectthetrialconclus
4、ions,besides,thequalityandquantityoftheFMusedinthecontrolarethemostimportant!Materials&methodsIBW:76.2±0.08gPositiveContorl:PeruFM（PFM）NegativeContorl:LocalFM（LFM）withhighVBN(148mg/kg)Alternativeproteinresources:Animalproteinblend(APB)PBM:MBM:BM:HFeM=40:35:20:5Substitutionlevel:20%、
5、40%、60%、80%ofPFMFormulationandproximatecompositionofexperimentaldiets (g.kg-1,wetbasis)IngredientsPFMLFMAPB20APB40APB60APB80Perufishmeal400.000.00320.00240.00160.0080.00Localfishmeal0.00500.000.000.000.000.00Animalproteinblend0.000.0080.00160.00240.00320.00Soybeanmeal210.00110.00210
6、.00210.00200.00200.00Wheatflour220.00220.00220.00220.00220.00220.00Fishoil50.0042.0046.3043.6049.3040.60L-lysine(50%)0.000.002.464.937.9110.34DL-methionine(98%)0.000.000.721.452.242.94L-threonine(98%)0.000.000.490.971.602.07Others120.00128.00120.00119.00119.00124.00Formulationandpro
7、ximatecompositionofexperimentaldiets (g.kg-1,wetbasis)ItemsPFMLFMAPB20APB40APB60APB80Proximateanalysis(wetbasis)Moisture(%)57.2555.4681.0486.0379.5979.75Ash(%)101.44119.2698.7697.2696.1497.10Crudeprotein(%)443.40449.80434.70434.40434.30436.00Grossenergy(MJ·kg-1)195.20197.10190.60190
8、.80189.70190.10Crud
