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ID:36852884
大小:1.47 MB
页数:49页
时间:2019-05-16
《北京荷斯坦牛乳成分的微卫星标记分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、四川农业大学硕士学位论文北京荷斯坦牛乳成分的微卫星标记分析姓名:王超申请学位级别:硕士专业:动物遗传育种与繁殖指导教师:李学伟;储明星20040501北京付斯岫小乳成分的微Ii星标记分析摘要奉研究分析了6个微卫星座位BM4505、BMl905、BMl443、BM415、BMl43和BM711在200头北京荷斯坦奶牛群体中的遗传变异(PIC、遗传杂合度、有效等位基因数和基因频率)。结果显示6个微卫星在群体中的PIC、遗传杂合度和有效等位基因数的范围分别为0.5339~O.8274、O.5624~0.8457和2.2851~6.4793。这6个微卫星座位均属于高度多念座位(
2、PIC>0.5)。对6个微卫星标记影uIq,“奶性状的分析结果表恻:微卫星BM4505座位刈叫二乳中乳脂率和干物质率影响显著(p‘O.05);微卫星BMl905座位对牛乳中蛋白率和乳糖率影响显著(p3、05座位上为225bp,在BM415座位上为168bp、170bp,在BM711座位上为190bp:影响蛋白率的增效基因在BMl905座位上为201b0,在BMl443座位上为163bp,在BM415座位上为168bp,在BMl43座位上为110bp;影响乳糖率的增效基因在BMl905座位上为201bp,在BM415座位上为168bp、170bp,在BMl43座位上为108bp;影u目干物质率的增效基因在BM4505座位上为225bp、239bp,在BM415座位L为168bp。关键词:奶牛,产奶性能,微卫星DNA,遗传变异北京付斯坦小乳j垃分的徽4、J星标记分析Anal5、ysisonmicrosatellitemarkersformilkcompositiontraitsinBeijingHolsteinsABSTRACTThegeneticvariation(polymorphicinformationcontent(PIC),heterozygosity,effectivenumberofallelesandgenefrequency)of6microsatelliteloci(BM4505,BMl905,BMl443,BM415,BMI43andBM711)wasanalyzedin200BeijingHolsteinsCOWpo6、pulations.rIheresultsshowedthatPIC,heterozygosityandeffectivenumberofallelesof6microsatellitelocirangefrom0.5339to0.8274,from0.5624toO.8457,from2.2851to6.4793.respectively.Allthe6microsatellitelociwerehighlypolymorphic(PIC>0.5).Effectsofsixmicrosatellitesonmilkproductiontraitswereanalyze7、d.Theresultsshowedthat:microsatellitelocusBM4505hadsignificanteffectsonmilkfatpercentageanddrymatterpercentage(p8、<0.05);microsatellitelocusBM415hadsignificanteffectsOilmilkfatpercentage,proteinpercentage,lactosepercentageanddrymatterpercentage(p
3、05座位上为225bp,在BM415座位上为168bp、170bp,在BM711座位上为190bp:影响蛋白率的增效基因在BMl905座位上为201b0,在BMl443座位上为163bp,在BM415座位上为168bp,在BMl43座位上为110bp;影响乳糖率的增效基因在BMl905座位上为201bp,在BM415座位上为168bp、170bp,在BMl43座位上为108bp;影u目干物质率的增效基因在BM4505座位上为225bp、239bp,在BM415座位L为168bp。关键词:奶牛,产奶性能,微卫星DNA,遗传变异北京付斯坦小乳j垃分的徽
4、J星标记分析Anal
5、ysisonmicrosatellitemarkersformilkcompositiontraitsinBeijingHolsteinsABSTRACTThegeneticvariation(polymorphicinformationcontent(PIC),heterozygosity,effectivenumberofallelesandgenefrequency)of6microsatelliteloci(BM4505,BMl905,BMl443,BM415,BMI43andBM711)wasanalyzedin200BeijingHolsteinsCOWpo
6、pulations.rIheresultsshowedthatPIC,heterozygosityandeffectivenumberofallelesof6microsatellitelocirangefrom0.5339to0.8274,from0.5624toO.8457,from2.2851to6.4793.respectively.Allthe6microsatellitelociwerehighlypolymorphic(PIC>0.5).Effectsofsixmicrosatellitesonmilkproductiontraitswereanalyze
7、d.Theresultsshowedthat:microsatellitelocusBM4505hadsignificanteffectsonmilkfatpercentageanddrymatterpercentage(p8、<0.05);microsatellitelocusBM415hadsignificanteffectsOilmilkfatpercentage,proteinpercentage,lactosepercentageanddrymatterpercentage(p
8、<0.05);microsatellitelocusBM415hadsignificanteffectsOilmilkfatpercentage,proteinpercentage,lactosepercentageanddrymatterpercentage(p
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