基因组改组技术选育嗜果糖、耐SO2葡萄酒酵母毕业论

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1、LANZHOUUNIVERSITYOFTECHNOLOGY毕业设计(论文)题目基因组改组技术选育嗜果糖、耐SO2葡萄酒酵母学生姓名学号专业班级指导教师学院答辩日期基因组改组技术选育嗜果糖、耐SO2葡萄酒酵母专业：学号：姓名：指导老师：职称：摘要以C-1、C-2、C-3、C-4四株酵母菌作为出发菌株，用紫外线和化学诱试剂进行诱变，分别用含高浓度SO2和果糖的培养基筛选，获得2株SO2耐受性提高的菌株C-2-4和C-4-2和2株果糖利用率提高的菌株C-1-8和C-3-1。再以这4株菌株作为正突变库，通过两轮的基因组改组，获得5株耐SO2性能和嗜果糖性能都较好的酵母菌株。其中第一轮

2、改组后的菌株A-2对SO2的耐受性与C-4-2相比提高59.7%，A-7利用果糖特性与C-1-8相比提高了20%。第二轮改组后的菌株B-1的耐SO2特性与C-4-2相比提高了14%，利用果糖特性与C-1-8相比提高了34%。关键词：葡萄酒酵母；基因组改组；耐SO2；嗜果糖；原生质体融合1AbstractByUVinducedmutagenesisandchemicalmutagenesismethodsofwineyeaststrainC-1,C-2,C-3andC-4,andscreeningonnutrientmediumcontaininghighconcentrati

3、onsofSO2andfructose,weobtained2SO2toleranceimprovedstrainsC-2-4andC-4-2andtwofructoseutilizationincreasingstrainsC-1-8andC-3-1.Wemadeuseofgenomeshuffingtothelibraryof4generateimprovedstrainsinthiswork.Weshuffedtwicetheseimprovedstrainbyprotoplastfusionandfinallyobtained5strainswithhigherSO2

4、toleranceandaddictedtofructose.TheSO2toleranceofthefirstroundofthereconstitutedstrainA-2improved59.7%comparedwiththeC-4-2,A-7featurestheuseoffructoseincreasedby20%comparedwithC-1-8.TheSO2toleranceofthesecondroundofGenomeShufflingstrainB-1hasa14%increasecomparedwiththeC-4-2,andtheuseoffructo

5、secharacteristicsincreasedby34%comparedwithC-1-8.Keywords:Wineyeast;GenomeShuffling;ResistancetoSO2;Addictedtofructose;Protoplastfusion2摘要.....................................................................................................................................................1Abs

6、tract................................................................................................................................................2一、综述.........................................................................................................................................

7、....5（一）葡萄酒酵母.....................................................................................................................5（二）葡萄酒酵母菌种的选育.................................................................................................51.自然选育......