GFP小鼠骨髓基质细胞诱导分化为神经细胞的实验研究

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1、第三军医大学硕士学位论文英文缩写一览表英文缩写英文全称中文全称GFPgreenfluorescentprotein绿色荧光蛋白BMSCsbonemarrowstromalcells骨髓基质细胞FBSfetalbovineserum胎牛血清RAretinoicacid维甲酸bFGFbasicfibroblastgrowthfacter碱性成纤维细胞生长因子DMSOdimethylsulfoxide二甲基亚砜BMEâ-mercaptoethanolâ巯基乙醇BDNFbrain-derivedneurotrophicfactor脑源性神经营养因子NGFner

2、vegrowthfactor神经营养因子GFAPgliadfibrillaryacidicprotein胶质纤维酸性蛋白NSEneuron-specificenolase神经元特异型烯醇化酶NF-200neurofilamentprotein200神经微丝蛋白200DABdiaminobezidin二氨基联苯胺1第三军医大学硕士学位论文Thestudyonneuralcellinducedanddifferentiatedthrough/bybonemarrowstromacellsinGFPmouseAbstractBonemarrowstromal

3、cells(BMSCs)arecellsgotridofhemopoieticstemcellsinmarrowcellofmatureindividual,whichisapprovedtobemulti-differentiationpotentialincludingtransformingintoneuralstemcell,anddifferentiationintoneursandneuroglialcell.InducingBMSCsintonervecellinvitroaimsatapplyingthattoclinicalandth

4、erapeutics.Underthiscondition,thecardinalwayisthatdrawingthematerialsfromBMSCs,andthencultureandproliferateandinduceitintoneuralcell,plantingdifferentiatedcellintobodyandsettingforthitsbiologicalroleandclinicaltherapy.Thecellstabilityofdifferentiatedphenotypeafterinductionislarg

5、elyunknown.Objective:StudyingmethodsofdetachmentandcultureinBMSCsinvitro,determiningtheoptimalschemthatBMSCsdifferentiateintoneuralcellsandobservingthephenotypestabilityininduced-BMSCsaimatapplicationofBMSCinfuture.Methods:(1)BMSCswereproliferatedinvitroafterseperatedandcultured

6、theminGFPmice.(2)theninducethembybasicfibroblastgrowthfactorb(bFGF),retinoicacid(RA),-mercaptoethanol(BME),brain-derivedneurotrophicfactor(BDNF),nervegrowthfactor(NGF)anddimethylsulfoxid(DMSO).ComparetheroleofRAand/orBMEatdifferentconcentrationininductionprocess.TheratiowhichBMS

7、Csdifferentiatedintoneuralcellwasexaminedthroughneurofilamentprotein200(NF-200),gliadfibrillaryacidicprotein(GFAP),neuronspecificenolase(NSE)andfibronectinbyimmunofluorescenceandimmunohistochemistryassays.Inducerandinducedconcentrationwaschoosed.(3)theproportionthatBMSCinGFPmice

8、differentiatedintoneuralcellwasdeterminedbyimmu