提高毛细管电泳分离和光学检测效能的途径及其应用研究

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1、中国科学院大连化学物理研究所博士后士学位论文提高毛细管电泳分离和光学检测效能的途径及其应用研究姓名：方梅申请学位级别：博士后士专业：分析化学指导教师：许国旺2001.9.1洲，’2确晕龟5国镑唁?务fc石／)／幺乏一驴穸事垆挈b、岁反够扩c。，伤羁锣衙乡心，f童≠夸@一多、恽，)z开留．舅欲毳，f2／之芝龟悯像／奄／句乙心-乏唿f勺毽．夸乏而矿留故一吲勿／芝乏弓旧锾皤／j乙萍匆诒-乌庀，匆重￡铷§垆f争乏t，／左闭。女墟1≈：竺堡垒唯!乱竺百一∑．璺f

2、起2叠譬专8；kSummaryCapillarydectrophoresis(CE)

3、isanewandexcellentseparationandanalyticalmethodItisworthtobefurtherresearchedInCEthedetectionsensitivityisamainproblembecausethesampleisoftendetectedincapillaryofnalTOWinterdiameterwithUV—VisdetectorItisnecessarytodevelopmethodstoimprovedetectionsensitivityofCEandputthem

4、intopracticalusesInparttamethodofoff-linesolid·phaseextractioncoupledtocapillaryelectrophoresis(inartuncoatedcapillaryusingboraxandboricacidinacetonitrife-water(40+60)asrunningbuffer)withUVdetection融254rimhasbeendescribedandusedfordirectdeterminationoftracebiomedicallyim

5、portantretinoicacidwithoutanyderivation．Upto107mol／LofretinoicacidinaqueoussolutionmaybedeterminedInpart2amethodfordeterminationofriboflavin(vitaminB2，VB2)inVB2tabletandcomplexVitaminB(VB)tabletsampleswasdevelopedThemethodissensitive(1imitofdetectionisO005_tg／mLofVB2Xsel

6、ective(coexistentVBl，Vt3。VBl：andVBcinthesamplesdonotinterferewiththedeterminationof、国2)’convenient(barecapillarywasusedwithoutprecoatingandnativefluorescenceofVB2wasmeasuredwithoutpreconcentrationorderivatization)，direct(withoutanyadditionalstep)andfast(forcompletionofth

7、edetermination，onlyca，5mineachtimewasconsumed)ThemethodhasbeenappliedtoVB2determinationinVB：tabletandcomplexVBtabletsampleswithsatisfactoryresults、Inpat’t3anewmethodfordeterminationoffolioacid《vitaminBc)inthepresenceofvitaminB12bycapillaryelectrophoresiswithUVphotodiodea

8、rrayhasbeendevelopedandhasbeenusedfordeterminationoffolicacidinitstabletandmixturescontainingfolicacidstandardandvitaminB{!tabletaswell豁folioacidandvitaminB12standardswithsatisfactoryresultswithoutinterferencefromvitaminB12，possibilityofsimultaneousdeterminationofVBcandV

9、Bj2intheirmixtureswasexaminedbyusingthemethodproposedAnalyticattimeismerelylessthan10rainKeywords：Retin