猪链球菌2型人源分离株截短的溶菌酶释放蛋白基因的克隆和原核表达

猪链球菌2型人源分离株截短的溶菌酶释放蛋白基因的克隆和原核表达

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1、J.AnimSci.2007.85:1750-1757.没有查到全文Periparturientcortisol,acutephasecytokine,andacutephaseproteinprofilesofgiltshousedingroupsorstallsduringgestationA.D.Sorrells*,,1,S.D.Eicher*,2,M.J.Harris,3,E.A.PajorandB.T.Richert*LivestockBehaviorResearchUnit,USDA-ARS,

2、PurdueUniversity,WestLafayette,IN47907;andDepartmentofAnimalSciences,PurdueUniversity,WestLafayette,IN479072Correspondingauthor:spruiett@purdue.eduUseofgestationstallsinporkproductionremainsacontroversialtopicinanimalwelfare.Immunestatusandmeasuresarefreq

3、uentlyusedtoassessstresslevelsandthuswell-beingofconfinedanimals.Theimportantwelfareissueofcloseconfinementamonggestatinggiltswastestedbyquantifyingcortisol,acutephasecytokine,andacutephaseproteinpro-filesbeforeandafterfarrowingofgiltshousedin2systems.Lan

4、dracexYorkshirecrossbredgiltshousedingroupsof4(group,n=8)inpens(3.9x2.4mwith4individualfeedingspaces,9.36m2totalor2.34m2/gilt)werecomparedwithgiltshousedinstandardindustrystalls(stall,n=16;2.2x0.6m,1.32m2/gilt).Floorswerefullyslatted,andasubstratewasnotpr

5、ovidedforeithersystem.Cortisolwasdeterminedfromsalivaond105ofgestation,1haftermovingthegiltsintofarrowingstalls(d111),and24hand7dafterfarrowing.Cortisolwasgreater(P=0.04)forgroupgiltscomparedwithstallgilts1haftermovingthemintofarrowingstallsand24hafterfar

6、rowing.Cortisolconcentrationsdecreased(P=0.001)overtime.LeukocytemRNAexpressionofIL-1,IL-1receptorantagonist,andtumornecrosisfactor-wasdeterminedbyquantitative,reversetranscriptionPCRond35,63,and91ofgestationand72hafterfarrowing.CytokinemRNAexpressionofpe

7、ripheralbloodmononuclearcellsdidnotdifferbetweenhousingsystemsforIL-1,itsreceptorantagonist,orfortumornecrosisfactor-.Acutephaseproteins,includingfibrinogen,haptoglobin,and1-acidglycoproteinweredeterminedforplasmasamplestakenatd35,63,and91ofgestationand72

8、hand14dafterfarrowing.Incontrasttocortisol,plasmafibrinogenconcentrationsincreased(P<0.005)overtime.Haptoglobindidnotdifferbetweentreatments(P>0.10).Stallgiltstendedtohavegreater(P=0.07)plasma1-acidglycoproteinconce

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