资源描述：
《猪链球菌2型人源分离株截短的溶菌酶释放蛋白基因的克隆及原核表达》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、J.AnimSci.2007.85:1750-1757.没有查到全文Periparturientcortisol,acutephasecytokine,andacutephaseproteinprofilesofgiltshousedingroupsorstallsduringgestationA.D.Sorrells*,,1,S.D.Eicher*,2,M.J.Harris,3,E.A.PajorandB.T.Richert*LivestockBehaviorResearchUnit,USDA-ARS,Purd
2、ueUniversity,WestLafayette,IN47907;andDepartmentofAnimalSciences,PurdueUniversity,WestLafayette,IN479072Correspondingauthor:spruiett@purdue.eduUseofgestationstallsinporkproductionremainsacontroversialtopicinanimalwelfare.Immunestatusandmeasuresarefrequentlyus
3、edtoassessstresslevelsandthuswell-beingofconfinedanimals.Theimportantwelfareissueofcloseconfinementamonggestatinggiltswastestedbyquantifyingcortisol,acutephasecytokine,andacutephaseproteinpro-filesbeforeandafterfarrowingofgiltshousedin2systems.LandracexYorksh
4、irecrossbredgiltshousedingroupsof4(group,n=8)inpens(3.9x2.4mwith4individualfeedingspaces,9.36m2totalor2.34m2/gilt)werecomparedwithgiltshousedinstandardindustrystalls(stall,n=16;2.2x0.6m,1.32m2/gilt).Floorswerefullyslatted,andasubstratewasnotprovidedforeithers
5、ystem.Cortisolwasdeterminedfromsalivaond105ofgestation,1haftermovingthegiltsintofarrowingstalls(d111),and24hand7dafterfarrowing.Cortisolwasgreater(P=0.04)forgroupgiltscomparedwithstallgilts1haftermovingthemintofarrowingstallsand24hafterfarrowing.Cortisolconce
6、ntrationsdecreased(P=0.001)overtime.LeukocytemRNAexpressionofIL-1,IL-1receptorantagonist,andtumornecrosisfactor-wasdeterminedbyquantitative,reversetranscriptionPCRond35,63,and91ofgestationand72hafterfarrowing.CytokinemRNAexpressionofperipheralbloodmononuclear
7、cellsdidnotdifferbetweenhousingsystemsforIL-1,itsreceptorantagonist,orfortumornecrosisfactor-.Acutephaseproteins,includingfibrinogen,haptoglobin,and1-acidglycoproteinweredeterminedforplasmasamplestakenatd35,63,and91ofgestationand72hand14dafterfarrowing.Incont
8、rasttocortisol,plasmafibrinogenconcentrationsincreased(P<0.005)overtime.Haptoglobindidnotdifferbetweentreatments(P>0.10).Stallgiltstendedtohavegreater(P=0.07)plasma1-acidglycoproteinconce
