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1、SRG基因的克隆及原核表达物质分解清除.如果Dc内吞坏死物质过多,往往不能将其充分溶解吸收,这亦可导致DC的坏死或凋亡.以上不同培养时问的Dc在透射电镜下超微结构和功能状态的表现,证实利用连续贴壁法分离培养的细胞,具有Dc典型的结构与多种功能表现.【参考文献】【1]BanchereauJ,SteinmanRM.Dendriticcellsandthecontrolofim—mumty[J].Nature,1998,392:245～250.[2]NishiokaY,HuaW,NishimuraN,eta1.Geneticmedificatonofdendriticcellsandits
2、applicationforcancerimmunotherapy[J].JMedInvest,2002,49(1/2):1一l7.【3]lna~aK,InabaM,RomaniN,eta1.Generationoflargenumbersofdendriticcellsfrommousebonemarrowculturessupplementedwithgranulocyte/macrophagecolony—stimulatingfactor[J].JExpMed.1992.176(6):1693—1702.SRG基因的克隆及原核表达闰露',高萍,栗艳,鱼兵Cloningandp
3、rokoryoticexpressionofSRGMODERNONCOLOGY.Mar.2007,VOI.1.SailustoF,LanzavecchiaA.Eficientpresentationofsolubleantigenbyculturedhumandendriticcellsismaintianedbygranuloeyte/macrophagecolony—?stimulatingfactorplusintedeukia'-4anddownregulatedbytumornecrosisfactor[J].JExpMed,1994,179(4):1109～1117.Ko
4、idoS,HaraE.HommaS.eta1.Dendriticcellsfusedwithallo.geneiceoloreetalcancercelllinepresentmultiplecolorcctalcancer—specificantigensandinduceantitumorimmunity~instautolo-goustumorcells[J].ClinCancerRes,2005,11(21):7891～790o.张红梅,张利旺,贾军,等.肿瘤患者自体血浆诱导树突状细胞的实验研究[J].现代肿瘤医学,2005,13(6):740～743.张利旺,张红梅,贾军,
5、等.以AFP为靶点的肝癌树突状细胞免疫治疗的实验研究[J].现代肿瘤医学,2005,13(6):736—739.YANLu,GAOPing,LIYan,YUBing'DepartmentofPharmacy,CenterofRehabilitation,ngHospital,￡FourthMilitaryMedicalUniversity,Xitm710033;DepartmentObstetricsandGynecology,TangduHospital,FourthMilitaryMedicalUniversity,Xi"an710038;'OrthopedicsOncology
6、InstituteofChinesePLA,TangduHospital,theFourthMilitaryMedicalUniversity,Xitm710038,China.【Abstract】0bjective:Toclone,expressandidentifyhumanSRGgene.Methods:TotalRNAwasextractedfromhumanosteosarcomacellsandthefu11lengthcDNAofSRGwasobtainedbyRT—PCR.TheSRGgenewasclonedin.topGEM—T—Easyvectorandsequ
7、enced.ThenthegenewasinsertedtoBamHIandSalIsiteofpET一28(a+)expressionvectortoconstructtheexpressionvectorwhichwastransformedintoE.coilBL21.Aftertl1etransformedbacteriawereinducedatIPTGfor2—6htheexpressedproteinwasanalyzedbySDS—PAGE
