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ID:36567188
大小:37.00 KB
页数:11页
时间:2019-05-12
《青藤碱对糖尿病大鼠脑缺血再灌注损伤P选择素和细胞间黏附分子1表达的影响》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、青藤碱对糖尿病大鼠脑缺血再灌注损伤P-选择素和细胞间黏附分子-1表达的影响作者:周素娴 苏珂 于健 彭鹰 周燕 林枫 李浩【摘要】目的探讨青藤碱(sinomenine,Sin)对糖尿病大鼠脑缺血再灌注损伤P-选择素和细胞间黏附分子-1(ICAM-1)表达的影响。方法采用高脂高糖饮食加腹腔注射小剂量链脲霉素建立糖尿病大鼠模型,造模成功后将大鼠随机分为假手术组、缺血再灌注组、Sin低剂量治疗组和Sin高剂量治疗组。线栓法建立局灶性脑缺血再灌注模型。Sin低(30mg/kg),高剂量(60mg/kg)治疗组于术前30min分别给予大鼠腹腔注射。用免疫组化法观察缺
2、血90min再灌注24h大鼠额顶部皮质P-选择素和ICAM-1表达,进行2,3,5-三苯基氯化四氮唑(TTC)染色和HE染色观察脑梗死体积及病理形态学变化。结果①糖尿病大鼠脑缺血再灌注24h,P-选择素和ICAM-1表达均明显增加,与假手术组比较,差异具有显著性(均P<0.05);与缺血再灌注组比较,Sin高、低剂量治疗组均显著减少P-选择素和ICAM-1表达(均P<0.05),高、低剂量组之间差异亦具有显著性(P<0.05)。②Sin治疗组脑梗死体积较缺血再灌注组减小,高、低剂量组之间差异亦具有显著性(P<0.05)。③11Sin
3、高、低剂量治疗组脑组织缺血损伤病理学改变明显轻于缺血再灌注组,Sin高剂量治疗组缺血改变亦轻于低剂量治疗组。结论Sin对糖尿病大鼠缺血再灌注脑损伤具有保护作用,其机制可能与减轻脑缺血再灌注损伤阶段P-选择素和ICAM-1所介导的炎症反应有关。【关键词】缺血再灌注;P-选择素;细胞间黏附分子-1;青藤碱 Abstract:ObjectiveTostudytheeffectofsinomenine(Sin)onexpressionofP-selectinandICAM-1followingcerebralischemiareperfusion(I/R)inj
4、uryindiabeticrats.MethodsInthisexperiment,ratmodelofdiabetesmellituswasmadebyhighsucrose,fatdietandstreptozotioninjection.Diabeticratswererandomlydividedinto4groups:shamoperatedgroup,I/Rgroup,lowdoseSintreatedgroupandhighdoseSintreatedgroup.Thefocalmiddlecerebralarteryocclusion(MC
5、AO)modelwasmadebysuture-occludedmethod.Sinweregivenintraperitoneallytorats30minbeforefocalcerebralischemiaoperationrespectively.After90minMCAOfollowing24hofreperfusion,weinvestigatedtheexpressionofP-selectinandICAM-1withimmunohistochemistryinischemicfrontalandparietalcortex.HEstai
6、ningandTTCstainingwerealsoinvestigated.Results①Comparedwithshamoperatedgroup,expressionofP-selectinandICAM-1wasincreasedat24hofreperfusionintheischemicterritory(P<0.05).ComparedwithI/Rgroup,lowandhighdoseSintreatedgroupdose-dependentlyreducedtheexpressionof11P-selectinandICAM-1
7、(allP<0.05).②ComparedwithI/Rgroup,cerebralinfarctionvolumeinlowdoseSintreatedgroupandhighdoseSintreatedgroupwasdecreaseddose-dependently(allP<0.05).③ThechangeofischemicimpairmentinloworhighdoseSintreatedgroupwaslighterthanthatofIRgroup,andhighdoseSintreatedgroupwaslightertha
8、nthatoflowdoseSintreatedgroup.Con
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