断乳前丰富环境对幼鼠海马神经可塑性的影响

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1、上海交通大学硕士学位论文断乳前丰富环境对幼鼠海马神经可塑性的影响姓名：陆澄秋申请学位级别：硕士专业：儿科学（儿童保健）指导教师：沈晓明20080501上海交通大学硕士论文富环境干预HIBD大鼠的Morris水迷宫成绩，损伤后7天开始干预者显著优于损伤后14天；而损伤后早期增加了短暂抚触处理的大鼠，其Morris水迷宫成绩与单纯丰富环境干预的大鼠没有明显差异。结论：断乳前丰富环境促进幼鼠海马相关的学习记忆能力，改变海马CA1区锥体神经元结构和功能的可塑性，其机制可能是激活了海马MAPK通路，上调了即刻早期基因Arc的表达。缺氧缺血损伤后7天开始

2、丰富环境干预优于损伤后14天，而短暂的抚触不能增加丰富环境干预的疗效。关键词：丰富环境，海马，断乳前，HIBD，神经可塑性-VI-上海交通大学硕士论文IMPACTOFPREWEANINGENRICHEDENVIRONMENTONNEURONALPLASTICITYOFYOUNGRATSABSTRACTObjectivesTodemonstratetheeffectsofpreweaningenvironmentalenrichmentonthestructuralandfunctionalplasticityofthehippocampusi

3、nyoungrats,tofindthepossiblemolecularmechanism,andtodetectthemosteffectivetimeandthebestmodelofenrichedenvironmenttotreatHIBD.MethodsApreweaningenrichedenvironmentalmodelwasestablished.Openfield,novelobjectrecognition,passiveavoidanceandMorriswatermazetestswereperformedtoex

4、ploretheeffectsofpreweaningenrichmentonhippocampusdependentlearningandmemory.ThemorphologicalchangesinCA1areaofhippocampuswereobservedbyGolgiandNisslestaining.WealsodetectedLTPoftheSchaffer-CA1pathwayinhippocampus.Theexpressionofactivity-regulatedcytoskeletalprotein(Arc)-VI

5、I-上海交通大学硕士论文andtheactivationofERK1/2weredetectedbyWesternBlotting.WeestablishedHIBDmodelandcomparedtheeffectsofenrichedenvironmentstartedat7days,or14daysafterHIBDandneonatalhandlingontreatingHIBD.ResultsRatsexposedtoenrichedenvironmenthadgreaterperformancesinnovelobjectreco

6、gnition,passiveavoidanceandMorriswatermazeteststhanthecontrolgroup.BoththelengthofthedendritesofCA1pyramidneuronswasincreasedandLTPinSchaffer-CA1pathwaywasenhancedbytheenrichedenvironment,howeverenrichedenvironmentdidn’tchangethenumberofneuronsinCA1area.Westernblottingtests

7、howedthattheexpressionofArcproteinwasupregulated,andERK1/2wasactivatedbyenrichedenvironment.Forhypoxic-ischemicbraindamage,ratsexposedtoenrichedenvironment7daysafterHIBDhadbetterperformanceinMorriswatermazetestthanthoseofenrichedenvironmentstartedat14daysafterHIBD.However,r

8、atsexposedtobothenrichedenvironmentandbriefneonatalhandlingdidn’tperformbetterthan