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ID:36467244
大小:2.50 MB
页数:97页
时间:2019-05-10
《米糟蛋白提取及酶法制备抗氧化活性肽及降血压活性肽的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、浙江大学博士学位论文米糟蛋白提取及酶法制备抗氧化活性肽及降血压活性肽的研究姓名:玄国东申请学位级别:博士专业:食品科学指导教师:何国庆20051101浙江大学博士学位论文10mg瓜g组和20mg/l(g组在给药4个星期后,sHR的血压分别平均F降了1688兀1mHg和26.22mmHg;米糟蛋白酶解物不仅具有促进sHR生长作用,而且sHR的心率没有任何影I晌。关键词:大米蛋白,血管紧张素转换酶抑制肽(AcEI),胰蛋白酶,抗氧化活性肽0浙江人学媾i:学位论文AbstractThetechnologiesoptim
2、umconditionsofexlractingproteinfromricedr。gs(RD),ric。p
3、otejnmodifyiog,extracIinga11tioxidatjVeactiVitypeptidea11dangiotensinI-converli“genzyme(AcE)inhibitorypeptidetowerestudied.EnzymaIicmelhod,alkaljnemethod,gelatjnnllration,highperfornlanceliquidc11romatogr
4、aphy(HPLC)andmassspectmm(Ms)wereemployedintheresearch.Theoptimumconditionsofex帆Kti89proteinfromRDwi也enZymaticmethodw盯eeD2ymedosage【E】/【S】_1%,ratioofrawmaterialtowater(R,w)1:4,pH=8.O,temperature=65℃,6me=7h.undertlleseconditions,extractiVerateofriceproleinwas62
5、.6%,a11dtheconcentraⅡ仰ofproteinintheextractwas84.O%.TheoptimumconditioⅡsextrac血培proteinfmmRDwithalkalinememodwerealkaliconcentration0_8%,R,W1:6,tempemture=65℃,time=6h.Undertheseconditions,extractivefale0friceprotejnwas76.2%,a11d【11econceDtra虹on0fproteinjntlle
6、exiractwas80.O%.TherewasevidentimproVemenIinlhedissolVabmtyJemlllsifiabnityandfoamabilityofproIeillexIracted丘DmRDafteritsmodific撕onw汕alcaIase.hl4%hydroIysatesneutraIsoIution,IhenilrogensolubilityiⅡdex(NsI),emulsi蛐唱abm‘弘f0帅abili‘ywere95%,55%,70%respectively1me
7、hydmlysateswhichderiVedfrDm血ehyd帕lyzationofRDpmtcinwi血alcalasepossesscdnotoIllyreducibilily,butthepowerofsca№ngiⅡgDPPHradicle.ThecapabniIyofscaⅣengingDPPHradjclewascomparedamonghydrolysales,teap01”henoI,bu哼latedhydr01ylanisole(BHA)柚dVjtaminC.TheresuItshowedma
8、tlhehydrolysateshadmerelyparallelpowertoscavcⅡgeDPPHmdidew“hBHA.WhenthereducibdityasresponseValue,responsesu—hceoptiIIlizationexperhentSwereperfonned.The佗sultsshow酣mattheoptiInalc蚰ditionsforextractingantioxidativeactivitypeptideswercasfoUows:pH=7.74,temperatu
9、re=61.5℃,Tatioofwatertomaterial5.23.1mehydrolysatesbytrypsinwerefoundtohaVethehi曲esIiIlhibitorya“vitytoACEaf【erthcactiVitycompareofhy出Dlysatesbyneutrase,acidpmte雒e,F1avou工zyme,alcaJase,pe
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