诱导悬浮培养东北红豆杉细胞凋亡过程蛋白表达差异研究

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1、天津大学硕士学位论文诱导悬浮培养东北红豆杉细胞凋亡过程蛋白表达差异研究姓名：赵红申请学位级别：硕士专业：生物化工指导教师：元英进20030101ABSTRACTApoptoticsystemsinducedbydifferentelicitorsandstudymethodsof2．DEandmassspectrometryhadbeensetuptostudythedifferentproteinsexpressionofapoptoticcellinsuspensionculturesofTaxuscuspidata．ThatthelmMCe”，SA(O．375mM)

2、andH202(O．012％)togetherCOuldinducecellapoptosiswasconfirmedhythemethodsoffluorescencemicroscopeandDNAelectrophoresis．Theresultof2-DgelelectrophoresisshowedthatisoelectficpointsandmolecularmassesofproteinsfromTaxuscuspidatacellvariedmainlybetweenpH4．0·8，0and40—80kDa．respectively．Difference

3、inproteinexpressionwasobservedbetweenapoptoticcellsandthecontr01．ThirteenproteinspotswereobservedonlyexistedintheCe4*-treatedsystems；tenofproteinspotswereobservedonlyintheSA-H202·treatedsystems．Besides，somecommonspotswhosevolumeswerereducedintwoapoptoticsystemsweTeobserved，Itsuggestedthat

4、theseproteinsmightrelatetotheinductionorirdfibitionofapoptosis．FromtheanalysisresultofMALDI—TOF—MS，we1earnedthatthereweresomeproteinswhosevoiumesincreasedintreatedcellssuchasCytochromecBiogenesisprotein、GlutathioneS—transferaseGST22、Catalase2andPathogenesis．relatedprotein．whilethevolumeof

5、LEAFY／FLORJcAuLAhomologprotein(MADSboxcontainingprotein)reducedintwotreatedsystems．ItsuggestedthatCytochromeCBiogenesisproteinmightplayakeyroleintheincitationofapoptosis；GlutathioneS．transfe：rageGST22andCatalase2mightberelatedtotheprotectionprocess．Moreover，theMADSboxcontainingproteinmigh

6、tbeallimportantproteinintheinhibitionofapoptosisinsuspensionculturesofTaxuscuspid口胁．AfterelicitationbySA—H202，02一’productionwerepromotedincellsandtheactivityoftheenzymesuch站SOD、CAT、APXintheculturefluctuatedatdifferenttime．ThesesuggestedROSmightplayallimportantroleintheprocessofapoptosis．K

7、eywords：Taxuscuspidata，Ce4+，salicylicacid，hydrogenperoxide，apoptosis，2-Dgelelectrophoresis，massspectrometryII独创性声明本人声明所呈交的学位论文是本人在导师指导下进行的研究工作和取得的研究成果，除了文中特别加以标注和致谢之处外，论文中不包含其他人已经发表或撰写过的研究成果，也不包含为获得叁壅盘望或其他教育机构的学位或证书而使用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了谢意。学位论