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1、JournalofZhejiangUniversity-SCIENCEB(Biomedicine&Biotechnology)ISSN1673-1581(Print);ISSN1862-1783(Online)www.zju.edu.cn/jzus;www.springerlink.comE-mail:jzus@zju.edu.cnClodronate-superparamagneticironoxide-containingliposomesattenuaterenalinjuryinratswithsevereacutepancreatitis*Sheng-chunDANG
2、†,Yan-huaZENG,Ping-jiangWANG,Bao-dingCHEN,Rong-fangCHEN,ArunKUMARSINGH,PankajKUMAR,ShuFENG,LeiCUI,HaoWANG,Jian-xinZHANG†‡(DepartmentofGeneralSurgery,theAffiliatedHospitalofJiangsuUniversity,Zhenjiang212001,China)†E-mail:dscgu@163.com;zhangjx@ujs.edu.cnReceivedSept.10,2013;RevisionacceptedNov
3、.11,2013;CrosscheckedMay20,2014Abstract:Backgroundandobjective:Ithasbeenshownthatmacrophagesplayanimportantroleinthedevelopmentofsevereacutepancreatitis(SAP),andeventuallyleadtomultipleorganfailure(MOF).Clodronate-liposomeselec-tivelydepletedmacrophages.Thisstudywastoinvestigatetheroleofrena
4、lmacrophageinfiltrationinacuterenalinjuryinratswithSAPandtoevaluatethepotentialofsuperparamagneticironoxide(SPIO)-enhancedmagneticresonanceimaging(MRI)fordiagnosis.Methods:SuperparamagneticFe3O4nanoparticleswerepreparedbychemicalcoprecipitation.SPIO-liposomesandSPIO-clodronate-liposomeswerep
5、reparedbythethinfilmmethod.SAPmodelswerepreparedbyinjectionofsodiumtaurocholateintothesubcapsularspaceofratpancreas.Sprague-Dawleyratswererandomlydividedintoacontrolgroup,SAPplusSPIO-liposome(P)group,andSAPplusSPIO-clodronate-containingliposome(T)group.KidneyinjurywasevaluatedbyT2-weightedMR
6、Iscan.Thelevelsofserumamylase(SAM),bloodureanitrogen(BUN),andserumcreatinine(SCr)weremeasuredbyanautomatedenzymaticmethod.Serumtumornecrosisfactor-α(TNF-α)wasmeasuredbyenzyme-linkedimmunosorbentassay(ELISA).Pathologicalchangesinthepancreasandkidneywereobservedusinghematoxylinandeosin(H&E)sta
7、ining,whilecellapoptosiswasdetectedwithterminaldeoxynucleotidyltransferasedUTPnickendlabeling(TUNEL)staining.Inaddition,themacrophagemarkers(CD68)oftherenaltissueweredetectedwithimmunohistochemistry.Results:Thepathologicalchangesinthepancreasandkid
