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1、黄曲霉毒素B1酶联免疫竞争检测法Hangzhou,Sept.24,20021酶联免疫竞争法工作原理Hangzhou,Sept.24,20022样品Hangzhou,Sept.24,20023样品黄曲霉毒素B1分子Hangzhou,Sept.24,20024微孔板Hangzhou,Sept.24,20025微孔表面情况AFB1抗体Hangzhou,Sept.24,20026标准品Hangzhou,Sept.24,20027标准溶液Hangzhou,Sept.24,20028酶标抗原Hangzhou,Sept.24,20029酶标抗原酶AFB1Hangzhou,
2、Sept.24,200210标准品,样品和酶标抗原加进微孔Hangzhou,Sept.24,200211样品酶标抗原样品孔先加后加Hangzhou,Sept.24,200212标准品酶标抗原标准孔先加后加Hangzhou,Sept.24,200213孵育30分钟抗原与抗体结合Hangzhou,Sept.24,200214微孔清洗Hangzhou,Sept.24,200215洗涤5次，间隔2分钟Hangzhou,Sept.24,200216加入酶催化反应底物Hangzhou,Sept.24,200217加入反应底物A和B孵育15分钟Hangzhou,Sept.
3、24,200218显色Hangzhou,Sept.24,200219Hangzhou,Sept.24,200220反应孔Hangzhou,Sept.24,200221阴性对照孔Hangzhou,Sept.24,200222阴性对照孔=没有黄曲霉毒素B1分子与酶标抗原竞争所有抗体都与酶标抗原结合Hangzhou,Sept.24,200223阳性对照孔Hangzhou,Sept.24,2002241ng/ml标准孔=黄曲霉毒素B1分子结合到抗体上剩余的抗体再与酶标抗原结合Hangzhou,Sept.24,200225阳性样品酶标抗原结合得越少，颜色越浅Hangz
4、hou,Sept.24,200226加入终止液，反应液由蓝色变成黄色，上酶标仪读取数据，画标准曲线Hangzhou,Sept.24,200227调零孔阴性孔0.1ng/ml0.5ng/ml1ng/ml5ng/ml10ng/ml酶联免疫竞争法标准曲线0.1110ng/mlO.D.Hangzhou,Sept.24,200228不同温度条件下的标准曲线0.1110ng/mlO.D.调零孔阴性孔0.1ng/ml0.5ng/ml1ng/ml5ng/ml10ng/mlHangzhou,Sept.24,200229以相对吸光度值为纵坐标三种不同温度下的标准曲线Y=A/A阴
5、性孔0.1110ng/ml100%50%调零孔阴性孔0.1ng/ml0.5ng/ml1ng/ml5ng/ml10ng/mlHangzhou,Sept.24,200230
