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1、基因枪介导灯盏花遗传转化及几个影响因素的研究?1076?中草药ChineseTraditionalandHerbalDrugs第37卷第7期2006年7月题将结合matK基因作进一步研究.再次是测序与克隆技术问题,国内普遍采用的是PCR产物直接测序,由于PCR产物浓度低,易受污染,给测序反应和读序工作造成了一定困难.本课题将经PCR获得的目的基因用大肠杆菌克隆后分离,净化,得到的目的基因不仅浓度高,而且污染少,测序,读序效果好,成功率显着提高,为目前日本,欧美等国家普遍采用的方法.,值得推广.References::1GrogoryMP.DouglasES,Pamel
2、aSS.Higherlevelrela—tionshipsofApiales(ApiaceaeandArahaceae)basedonphy—logenelicanalysisofrbcLsequencesEJ].AmJBot,1996,83(4):99—515.-2]GregoryMP,DouglasES,PamelaSS.ClarificationoftherelationshipbetweenApaceaeandAraliaceaebasedonmatKandrbcLsequencedata[J].AmJBot,1997,84(4):565—58O.:3:Ste
3、phenRD,DeborahKD.AmolecularphylogenyofApi—aceaesubfamilyApioideae:evidencefromnuclearribosomaDNAinternaltranscribedspacersequences[J].AmJBot,1996,83(2):234-251.4]StephenRD,SeemantiR,DeborahSKD,eta1.MolecularsystemlaticsofApiaceaesubfamilyApioideaephyolgeneticanalysisofnuclearribosomalDN
4、AinternaltranscribedspacerandplastidrpoClintronsequences[J].AmJBot,1998,85(4):563—591.:5]GregoryMP,StephenRD.MajorlineageswithinApiaceaesubfamilyApioideae;AcomparisonofchloroplastreserictionsiteandDNAsequencedata[J].AmJBot,1999,86(7):1014—1026.:6]StephenRD,DeborahSKD,MarkFW.Aphylogenyof
5、thefloweringplantfamilyApiaceaebasedonchloroplaseDNArpl16andrpoC1intronsequences:TowardsasupragenericclassificationofsubfamilyApioideae[J].AmJBot,2000.87(2):273—292.:7:BaldwinBG,SandersonMJ,PorterJM,eta1.TheITSre—gionofnuclearribosomalDNA:avaluablesourceofevidenceonangiospermphylogeny[J
6、].AnnMoBotGard,1995,82:47—77.[8][9][1O][11][12][13][14][15][16][17][18][19]DoyleJI,DoyleJA.ArapidDNAisolationprocedureforsmallquantitiesoffreshleafmaterial[J].PhytochemBull,1987,19:11—15.WhiteTJ,BrunsT,LeeS,eta1.AmplificationandDirectSequencingofFungalRibosomalRNAGenesforPhylogenet—icsr
7、M].California:AcademicPress,1990.SangerF,NicklenS,CoulsonSR.DNASequencingwithchain—terminationinhibitors[J].ProcNatAcadSciUSA,1977,74:5463—5467.TheJapaneseBiochemicalSociety(JBS):PracticalBiochem—istryExperimentation[M].Taikyo:ChemicalCommissionofTaikyo.1995.DowineSR,OlysteadRG
