大鼠肾脏缺血再灌注损伤过程中水通道蛋白-2的表达变化

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1、授予单位代码10089学号或申请号13802K於(E搿走§HebeiMedicalUniversity硕士学位论文在职科学学位大鼠肾脏缺血再灌注损伤过程中水通道蛋白-2的表达变化学位申请人：梁慧导师：赵松教授专业：病理学与病理生理学二级学院：基础医学院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独1兀成。本学位论文研究所获的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文相关的论文，第一署名单位为河北医科大学，试验材料、原始数据、申报的专利等知识产权均归河

2、北医科大学所有。否则，承担相应的法律责任。研究生签名导师签章Q二级学院领导签1^1;年么月A日河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果，指导老师对此进行了审定。本论文由本人独立撰写，文责自负。年6月/曰目录中文摘要·············································································1英文摘要·······································

3、······································4英文缩写·············································································8研究论文大鼠肾脏缺血再灌注损伤过程中水通道蛋白-2的表达变化前言·············································································9材料与方法······················································

4、··············12结果·············································································21附图·············································································24附表·············································································31讨论·····························

5、················································33结论·············································································37参考文献·······································································38综述水通道蛋白2的研究进展···············································42致谢··················

6、·································································51个人简历·············································································52中文摘要大鼠肾脏缺血再灌注损伤过程中水通道蛋白-2的表达变化摘要目的：肾脏作为高灌注器官，对缺血非常敏感，容易发生缺血再灌注损伤（ischemicalreperfusioninjury,IRI）。肾IRI是缺血性急性肾功能衰竭（acuterenalfailure,ARF）的重要损

7、伤环节，其损伤的病理生理机制非常复杂，目前对肾IRI病理生理机制的研究主要集中在肾小管上皮细胞的凋亡和坏死、自由基的损伤作用、钙超载、能量代谢障碍、炎性介质和粘附分子、细胞因子等方面，而对肾脏存在的水通道蛋白（AQPs）在这一病理生理条件下的作用认识不足。为此，本研究通过建立大鼠的肾脏IRI模型观察其AQP2表达变化与肾功能的关系，探讨ARF时多尿与AQP2表达间的相关性，为临床防治ARF提供理论依据。方法：选取50只健康成年的雄性SD大鼠，分为实验组和对照组，实验组通过腹腔分离右侧肾蒂用无损伤动脉夹夹闭，45mi