兔smscs的体外分离培养及细胞标记的实验研究

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1、山西医科大学硕士学位论文分类号：R684.1学校代码：10114密级：学号：2012200555兔SMSCs的体外分离培养及细胞标记的实验研究TheStudyonIsolation,CultureandtheCellLabelingofRabbitSynovialMesenchymalStemCellsinvitro研究生：毕声荣指导教师：余方圆副教授专业名称：外科学研究方向：关节软骨损伤与修复学位类型：专业学位所在学院：第二临床医学院中国山西二〇一五年五月二十日目录中文摘要.......................................

2、.........................................................................................I英文摘要.............................................................................................................................III常用缩写词中英文对照表..............................................

3、....................................................V前言...................................................................................................................................11材料与方法......................................................................................

4、...............................31.1材料.............................................................................................................................31.2实验方法.............................................................................................................

5、........61.3统计学分析...............................................................................................................102结果...........................................................................................................................112.1SMSCS的分离、培养.......

6、........................................................................................112.2SMSCS的纯化...........................................................................................................122.3SMSCS的增殖特性（CCK-8法）................................................

7、..........................132.4EGFP基因/SPIO双标记SMSCS细胞....................................................................142.5双标记SMSCS向成软骨、脂肪及成骨细胞定向诱导分化.................................162.6SMSCS细胞EGFP/SPIO双标记后行细胞增殖状态检测（CCK-8法）.............173讨论...................................

8、...............................................