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ID:35167157
大小:2.06 MB
页数:48页
时间:2019-03-20
《mg63骨肉瘤细胞中的inos的信号调控机制》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、分类号:R681学校代码:10114密级:学号:2012100054MG63骨肉瘤细胞中的iNOS的信号调控机制SignalregulatorymechanismsofiNOSinMG63osteosarcomacells研究生:王小强指导教师:赵斌主任医师专业名称:外科学研究方向:脊柱外科学位类型:学术学位所在学院:第二临床医学院中国山西二〇一五年五月二十日目录中文摘要...............................................................................................
2、.............................................................I英文摘要.........................................................................................................................................................III常用缩写词中英文对照表.................................................
3、...........................................................V前言.............................................................................................................................................11材料与方法........................................................................
4、.......................................................31.1细胞株...............................................................................................................................31.2主要试剂.....................................................................................
5、......................................31.3自配试剂...........................................................................................................................41.4主要仪器设备.......................................................................................................
6、............41.5细胞培养...........................................................................................................................41.6Ag溶液和MAZ-51溶液的配置........................................................................................61.7硝酸酶还原法测定NO浓度...............
7、..............................................................................61.8PT-PCR分析各组中MG-63细胞中iNOSmRNA的表达情况............................................71.9Westernblot方法检测各组iNOS蛋白表达..................................................................91.10统计学方法...................
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