tert促耳蜗前体细胞增殖及其机制研究

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1、分类号UDC密级TERT促耳蜗前体细胞增殖及其机制研究宋勇莉培养类别全日制学位类型专业学位一级学科(专业类)临床医学二级学科(专业)耳鼻咽喉科学研究方向耳聋的基础研究指导教师邱建华教授（主任医师）培养单位西京医院耳鼻咽喉头颈外科二O一五年五月第四军医大学博士学位论文第四军医大学博士学位论文目录缩略语表·······························································································1中文摘要····································

2、···························································2英文摘要·······························································································7前言·····························································································13文献回顾··························

3、···································································15一、毛细胞损伤与再生······································································15二、耳蜗前体细胞研究进展································································16三、端粒及端粒酶研究进展··············································

4、··················20正文·····························································································23实验一新生大鼠耳蜗前体细胞的分离、培养··········································231材料···························································································232方法········

5、···················································································253结果···························································································314讨论···························································································38实验二TERT在耳蜗

6、发育过程中及耳蜗前体细胞中增殖的表达···················401材料···························································································402方法···························································································423结果·····················································

7、······································474讨论···························································································53实验三Ad-TERT-EGFP的构建和大鼠耳蜗前体细胞的转染························561材料···················································································