凋亡抑制蛋白c-flip促进肝癌细胞葡萄糖代谢机制的研究

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1、分类号R735.7UDC577密级公开凋亡抑制蛋白c-FLIP促进肝癌细胞葡萄糖代谢机制的研究雷世雄培养类别全日制学位类型专业学位一级学科(专业类)临床医学二级学科(专业)外科学研究方向肝癌发病机制的基础研究指导教师杜锡林副教授（副主任医师）培养单位唐都医院普外科二O一五年五月第四军医大学硕士学位论文目录缩略语表······························································································1中文摘要··············

2、················································································4英文摘要······························································································7前言························································································

3、·····11文献回顾·····························································································12正文·····························································································23实验一c-FLIP对肝癌细胞糖酵解糖及葡萄糖摄取的影响·····························23引言·······

4、······················································································231材料···························································································241.1试剂·················································································

5、······241.2细胞系和动物···········································································241.3仪器·······················································································251.4慢病毒载体信息········································································

6、251.5引物······················································································271.6siRNA序列··············································································272方法··················································································

7、·······272.1细胞培养·················································································272.2慢病毒感染及筛选·····································································272.3细胞转染·················································································2

8、82.4免疫印迹实验（WesternBlot）·····················································282.5实时定量PCR（qRT-PCR）·············