mir-200azeb2调控肝癌干性样细胞恶性表型转化的机制研究

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1、分类号R735.7UDC616-006密级公开MiR-200a/ZEB2调控肝癌干性样细胞恶性表型转化的机制研究杨西胜培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)外科学（普外）研究方向肝癌分子机制研究指导教师李海民教授（主任医师）培养单位西京医院普外科二O一五年五月第四军医大学硕士学位论文目录缩略语表·······························································································1中文摘要·

2、······························································································3英文摘要·······························································································6前言·····································································

3、··························9文献回顾······························································································10正文······························································································26第一部分MiR-200a在肝癌组织中的表达及其与临床病理的关系··········

4、········261材料····························································································261.1实验标本················································································261.2实验细胞·······························································

5、·················261.3实验试剂：·············································································261.4主要仪器················································································272方法····································································

6、························282.1细胞培养及传代·······································································282.2Real-timePCR定量····································································282.3免疫组化染色·····························································

7、·············292.4统计学方法·············································································293结果····························································································303.1MiR-200a在肝癌组织及细胞系中的表达·········································30

8、3.2MiR-200a与肝癌患者临床病理的关系············································303.3MiR-200a与侵袭转移和预后的关系···············································