泰安地区鸡源大肠杆菌的分离鉴定与耐药性分析

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1、、＇、＊－‘＇’ｉｖ．护；ｒ、心产ｖ：Ｔ＾ｆ？，．—？‘分类号。、：Ｓ８５：、堪心：授予学位单位代码：１０４３４．；於洩謀托Ｖ讀■：．．‘…．－：Ｚ２１１３８．與群巧：编０００；；／祝互ｆ，号茲；、、．、．＇＇．．．？术－：＇＇＾．＾蝴－：ｖ护斯，巧、八＇＂Ｉ薄、詞警：隱讚麵氣：賴＇邊靡．―調．亂义峡化’Ｉ．＇’斷：Ｗ寒巧乂八參处．今：．和為璋〇，＇哨雜鄉‘．＇’、．．＂．’．，．＇巧气、：．’知：巧＜ｖ，／淹聲扣、ｃ＇－－．ｆｒ．雜部说，：Ｔ囑、爾津专业＇学准论八

2、．＇．．切：、：．：：：：．；…？幣．；成又、．．—兵／，．、＇、‘＇、＾１乃．．．，Ｖ．戚押＇背基‘＇，芯、、Ａ－、：，？，＇？山、．、，‘氏义＞１—／。，ＶＩ：；焉，；泰安地区鸡源犬肠杆菌的分离鉴定与耐药性分篇吏萌：■．‘．’＇；／；，若結？Ｉｓｏｌａ村ｏｎａｎｄＤｒｕｇＲ的ｉｓ化ｎｅｅＡｎａｌｓｉｓｏｆＡｖｉａｎＰａ化ｏｅｎｉｃ！沒；％＾ｙｇ片丘ｃｏＫｉｎＴａｉａｎ，ＳｈａｎｄｏｎｇｐｒｏｖｉｎｃｅＴ媒發論、為备一＂＇．．、．’、

3、巧、，苗吟；＞＇＾；；公載據溝苗；／或．．；．，．．－％：－，．暫巧尽声’；：我：，，说＂念式ｉ無％如芯讓、令磕齡、．．、‘、，冷＾．冷位：击＾鞍訪类别兽医硕巧辩批：寻或；马努＾、蒜．歎這．：、．｜’■一听Ｖ挺错餘综：；ｒ典今记靜：．‘化論接Ｍ婦，八．…‘－．！预防兽巧域度＞学苗却妒、：二Ｖ六说綠＂｜．‘，沪．、；、．．．，￣茄；；ｎ＇＂，ｙＴ．八，，户去為知、．‘研：Ｖ一究方向动抵检疲与品松验食；刊緣知Ｙ、娘鸿请，於’’＾１．子皆：持．作苗．学院动物技‘科枯這学韓裤沪遠爭．指言顺微．师崔

4、甚制：．、，巧逵＾；．＇’？＇？一＊，－＂＊一＇？’－、＇、．一、＇，＜－＞、？、－．心心、、２甲．＇’沪聲’１５１２年；月巧；；游祭次、為＇寵？少、ｉ巧濟ｆ，．終Ｍ：．油：韓觸巧４編、＞筑把彎／满證；捉苗屬论文提交日期：2015.10.12论文答辩日期：2015.12.03学位授予日期：2015.12学科门类：兽医硕士答辩委员会主席：高迎春缩略词英文缩写英文全称中文名称APECAvainapathogenicEshcercihiacoli禽病原性大肠杆菌bpbasepair碱基对CFUColonyFormi

5、neUnit菌落形成单位DNAdeoxyribonucleicacid脱氧核糖核酸dNTPeoxyribonucleosidetriphosphate脱氧核糖核苷三磷酸E.coliEshcercihiacoli大肠埃希氏菌EBEthidiumBrothide溴化乙锭hhour小时kbkilobase核酸分子中1000个碱基对LLiter升LBLuria-bertinimediumLB培养基minminute分钟mLMilliliter毫升PBSphosphatebuffersaline磷酸盐缓冲液PCRPolymerasechainreacti

6、on聚合酶链式反应r/minRoundperminute每分钟转数目录中文摘要······························································································································Ⅰ英文摘要·········································································································

7、·····················Ⅱ1前言·····································································································································11.1病原形态及染色特性·····································································································21.

8、2培养特性······································································