活性多肽的表达及功能研究.pdf

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1、分类号Q819学号14026013ＵＤＣ606密级公开工学博士学位论文活性多肽的表达及功能研究博士生姓名吴慧学科专业生物医学工程研究方向活性多肽工程技术研究指导教师张东裔教授国防科学技术大学研究生院二〇一七年四月ExpressionandfunctionalanalysisoftheactivepolypeptideCandidate：WuHuiSupervisor：ZhangDongYiAdissertationSubmittedinpartialfulfillmentoftherequirementsforthed

2、egreeofDoctorofEngineeringInBiomedicalEngineeringGraduateSchoolofNationalUniversityofDefenseTechnologyChangsha，Hunan，P.R.ChinaApril，2017国防科学技术大学研究生院博士学位论文目录摘要........................................................................................................

3、.......................iAbstract.......................................................................................................................iii第一章绪论.......................................................................................................

4、..............11.1蜘蛛毒素的简介及研究进展.............................................................................11.1.1毒素简介...................................................................................................11.1.2蜘蛛简介....................................

5、...............................................................11.1.3获取毒素的方法.......................................................................................31.2蛋白的表达及纯化研究进展.............................................................................31.2.1

6、蛋白的表达................................................................................................31.2.2蛋白的纯化................................................................................................51.3表达融合蛋白标签的选用.......................................

7、..........................................61.3.1HIS标签.....................................................................................................61.3.2GST标签.................................................................................................

8、...61.3.3Flag标签....................................................................................................61.3.4SUMO标签.......................................