tgf-β1、pdgf-b在tlr4信号通路中作用的研究

《tgf-β1、pdgf-b在tlr4信号通路中作用的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、分类号：S859.3密级：公开论文编号：2012021999贵州大学2015届硕士研究生学位论文TGF-β1、PDGF-B在TLR4信号通路中作用的研究学科专业：基础兽医学研究方向：动物药理学导师：王鲁教授研究生：张小艺中国﹒贵州﹒贵阳2015年5月ResarchonrolesofTGF-β1andPDGF-BontheTLR4signalingpathwaysMajor:AnimalpharmacologySupervisor:ProfessorWANGLuMDCandidate:ZHANGXiaoyiDepartmentofAnimalMedicine,Colleg

2、eofAnimalScience,GuizhouUniversity,Guiyang550025,PRC.ProgramSupportedbyNationalScienceResearchCouncilofChina(31470128；31260618；31060347)May,20151TGF-β1、PDGF-B在TLR4信号通路中作用的研究目录目录...............................................................................................................

3、.......2摘要......................................................................................................................4Abstract................................................................................................................66缩略语(abbreviations)...................

4、......................................................................8前言......................................................................................................................91材料.............................................................................................

5、...................121.1实验动物.............................................................................................121.2主要试剂.............................................................................................121.3主要实验仪器......................................................

6、...............................131.4主要溶液的配制.................................................................................132方法................................................................................................................152.1小鼠MECs的制备与提取..............................

7、..................................152.1.1混合酶消化法培养小鼠MECs..............................................152.1.2小鼠MECs的总RNA的提取...............................................152.1.3小鼠MECs总RNA反转录为cDNA...................................162.2实时荧光定量RT-PCR筛选TGF-β1、PDGF-B的siRNA最佳转染条