资源描述：
《Electrochemical investigation of the interaction between topotecan and DNA at disposable graphite electrodes.PDF》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、Bioelectrochemistry102(2015)21–28ContentslistsavailableatScienceDirectBioelectrochemistryjournalhomepage:www.elsevier.com/locate/bioelechemElectrochemicalinvestigationoftheinteractionbetweentopotecanandDNAatdisposablegraphiteelectrodesGulsahCongur,Ar
2、zumErdem⁎,FehmiMeseEgeUniversity,FacultyofPharmacy,AnalyticalChemistryDepartment,35100Bornova,Izmir,TurkeyarticleinfoabstractArticlehistory:Topotecan(TPT)isasemisynthetic,watersolubleanalogoftheplantalkaloidcamptothecinwhichhasbeenReceived13April2014
3、widelyusedforthetreatmentofovarianandcervicalcancers.ToobtainbetterunderstandingonhowitcanaffectReceivedinrevisedform9November2014DNAstructure,electrochemicalbiosensorplatformsfortheinvestigationofTPT-doublestrandedDNA(dsDNA)Accepted16November2014int
4、eractionweredevelopedforthefirsttimeinthisstudy.TheelectrochemicaldetectionofTPT,andTPT–Availableonline20November2014dsDNAinteractionwereinvestigatedatthesurfaceofpencilgraphiteelectrodes(PGEs)andsingle-walledcarbonnanotube(SWCNT)modifiedPGEsbyusingdif
5、ferentialpulsevoltammetry(DPV).ThechangesattheoxidationKeywords:TopotecansignalsofTPTandguaninewereevaluatedbefore/aftereachmodification/immobilizationstep.Anenhancedsen-Drug–DNAinteractionssorresponsewasobtainedbyusingSWCNT–PGEscomparedtounmodifiedPGE
6、swithresultinglimitsofdetec-Pencilgraphiteelectrodetion(LODs)forTPTas0.51μg/mL,0.45μg/mL,0.37μg/mL(130pmol,117pmol,96.5pmolina110μLsample,Singlewalledcarbonnanotubesrespectively)byusingelectrochemicallypretreatedPGE,unmodifiedPGEandSWCNTmodifiedPGE.Ina
7、ddition,Differentialpulsevoltammetryelectrochemicalimpedancespectroscopy(EIS)measurementswereperformedforthepurposeofmodificationofElectrochemicalimpedancespectroscopyPGEsbyusingSWCNTsandtheinteractionprocessatthesurfaceofSWCNT–PGEsbyevaluatingthechan
8、gesatthechargetransferresistance(Rct).©2014ElsevierB.V.Allrightsreserved.1.Introductionwithboththe−1(upstream)and+1(downstream)basepairs[11,12].TherehavebeensomereportsaboutdeterminationofTPTwhichNucleicacidrecognitiontechnologiescombinedwithelectroc
