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1、Protein&PeptideLetters,2008,15,000-0001TheInteractionbetweenCholesterolandHumanSerumAlbumina,abaaLiuPeng*,HeMinbo,ChenFang,LiXiandZhangChaocanabSchoolofSciences,WuhanUniversityofTechnology,Wuhan430070,China;DepartmentofCardiovascularDiseases,WuhanGeneralHosp
2、ital,Wuhan430070,ChinaAbstract:TheinteractionbetweencholesterolandHumanSerumAlbumin(HSA)wasstudiedbyfluorescencetechnique.AdditionofcholesterolcausesdecreasingofthefluorescenceintensityofHSAandthemechanismcanbeattributedtostaticquenching.Bothnegativeenthalpy
3、andentropychangeindicatethisbindingwasan“enthalpy-driven”reaction.Thenumberofbindingsiteanddistancebetweenresiduesandligandswerealsocalculated:n=0.98,r=3.84nm.UV–visspectrashowedHSAmoleculesunfoldedtosomeextentandthehydrophobicitywasdecreasedinthepresenceofc
4、holesterol.Keywords:Cholesterol,quenching,thermodynamicparameter,hydrophobicity.1.INTRODUCTION2.MATERIALSANDMETHODSHumanserumalbumin(HSA)isthemostabundantpro-2.1.Materialsteininthesystemiccirculationcomprising60%inplasmaHSAandcholesterol(Fig.1)wereboughtfrom
5、Sigma[1,2].ManyreportshaveshownthattherearetwomajorandBodiofTianjing,respectively.Trisbufferwithapurityligandbindingsiteslocatedinthesubdomainsof2Aand3Aofnolessthan99.5%andNaCl,HClwereallofanalyticalonHSA[3].Whileitsprincipalfunctionistotransportfattypurity.
6、TheHSAwasdissolvedinTris–HClbuffersolutionacids,itisalsocapableofbindinganextraordinarilybroad-1-1(0.05molLTris,0.15molLNaCl,pH7.4±0.1).Allso-rangeofdrugs[4].Muchoftheclinicalandpharmaceuticallutionswereusedwithdoublydistilledwater.interestintheproteinderive
7、sfromitseffectsondrugphar-macokinetics.HSAhasbeenimplicatedintransport,storage,CH3andmetabolismofmanymetalions,andhasmanyphysio-logicalfunctionsasthemajorconstituentofthecirculatoryCHCH33system[5,6].HSAoftenincreasestheapparentsolubilityofCH3hydrophobicdrugs
8、inplasmaandmodulatestheirdeliverytocellsinvivoandinvitro.CH3Cholesterolisapotentmodulatorofthebulkphysicalpropertiesofbiologicalmembranes[7,8]andthepresenceofcholesterolorcholesterol-richphospho
