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1、·1072·中草药ChineseTraditionalandHerbalDrugs第37卷第7期2006年7月·药材与资源·中日当归属药用植物ITS序列分析133221X赵国平,新关稔,石川隆二,钱三旗,章群,敖杰男(11暨南大学医学院,广东广州510632;21暨南大学生命科学技术学院,广东广州510632;31日本弘前大学农学生命科学部,日本青森03628561)摘要:目的建立当归属药用植物功效的分子系统学基础,为当归属药用植物功效的差异性提供分子依据。方法采用PCR技术与克隆技术相结合,获得ITS基因,进行测序,经Phylip软件统计
2、分析和分支分析,建立系统发育树,计算各类群间的遗传距离。结果当归属药用植物中ITS12518S2ITS2序列长度为600～629bp,去除序列长度为165bp的518S,ITS1+ITS2排序后的长度为468个位点。系统树将当归属药用植物分为两组,以独活、白芷为代表分别聚类,甘肃产岷当归Angelicasinensis不与上述任何一组聚类,却与欧当归Levisticumofficinale遗传距离相对较小。结论上述结果与当归属药用植物功效差异基本吻合,启示药用植物亲缘演化关系可能是研究药用植物功效规律的一条重要途径。关键词:当归属;基因转录
3、间隔区;遗传距离;功效中图分类号:R28217文献标识码:A文章编号:02532670(2006)07107205ITSSequenceanalysisofChineseandJapanesemedicinalplantsofAngelicaL11332ZHAOGuo2ping,NIIZEKIMinoru,ISHIGAWALiuni,QIANSan2qi,21ZHANGQun,AOJie2nan(11MedicalCollege,JinanUniversity,Guangzhou510632,China;21CollegeofLifeSci
4、enceandTechnology,JinanUniversity,Guangzhou510632,China;31FacultyofAgriculturalandLifeScience,HirosakiUniversity,Hirosaki03628561,Japan)Abstract:ObjectiveToestablishthebaseofmolecularsystem2theoryonmedicinalplantsofAngeli2caL1andtooffermoleculargistsforfindingtheefficacydi
5、fferenceofthem1MethodsThePCRtechniquewasutilizedtogetherwiththeclonetechniquetogettheITSgeneandmeasureitsgenicsequence1Statisti2calanalyzingandbranchanalyzingwerecarriedoutbyPhylipsoftwareandgeneticdistancedifferenceineverykindscanbecalculatedwithMEGA31ResultsITS12518S2ITS
6、2,belongstooneofmedicinalplantsofAngelicaL1,itssequencelengthextents600to629bp1Subtracting518S,itslengthis165bp,thelengthofITSis468bp1ThemedicinalplantsofAngelicaL1aredividedintotwogroupsbydendrogram1ThegeneticdistanceofA.sinensisfromGansuProvinceisobviouslydifferentfromth
7、eothermedicinalplantsofAngelicaL1,butthedistancedifferencecomparedwithLevisicumofficinaleissmaller1Conclu-sionItsuggeststhattheaboveresultbeidenticaltotheefficacyofmedicinalplantsofAngelicaL1ItmaybeanimportantreasonofexplainingwhytheefficacybetweenA1sinensisandothermedicin
8、alplantsofAn2gelicaL.issodifferent1Keywords:AngelicaL1;internaltranscribedspacer;geneticd
