二氮嗪预处理对大鼠脑缺血再灌注损伤后血脑屏障的保护作用分析

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1、·英文论著摘要·Researchontheprotectiveeffectofdiazoxidepretreat--．mentontheblood．brainbarrierofratsaftercerebralischemia／reperfusioninjuryobjeetiveTostudytheeffectanditsmechanismofdiazoxideontheblood-brainbarrier(BBB)ofratsaftercerebralischemia／reperfusion(I／R1injury．MethodsSixtyWista

2、rratswererandomlydividedintoshamoperationgroup、I／Rgroupanddiazoxidepretreatmentgroupsoflow、middle、largedose(5、10、20mg／kg)，TheI／Rmodelsofratswereperformedtoundergomiddlecerebralarteryembolismbythread．BBBpermeabilitywasestimatedbyEvan’S—blue(EB)dyeing，Transmissionelectronmicrosco

3、pe(TEM)Wasscheduledtoobservethemodificationofinterendothelialtightjunction(TJ)ofcapillaries，theexpressionofClaudin-5andZo-1ineveryratbraintissueswasdetectedbyimmunityhistochemistrytechnique．Resuits1．Comparedwithshamoperationgroup，ThepermeabilityextentofEBweresignificantlyincrea

4、sedbyI／Rwhichwasdistinctlyattenuatedinmiddleandlargedoseofdiazoxidepretreatmentrats，whilenoobviouschangeswerefoundbetweenFRandlowdosegroups．2．TEMshowedthatTJofthebraintissueopenedafterURinjuryandnosignificantopeningofTJWasobservedinmiddleandlargedoseofdiazoxidepreconditioninggo

5、ups．33．Comparedwithshamoperationgroup，theexpressionofClaudin一5andZo一1inthebraintissueoftheI／Rgroupwereapparentlydecreased(P

6、noobviousdifferencesbetweenlowdosegroup(5mg／kg)andtheI／Rgroup(P>0．05)．ConclusionPreconditioningofischemia／reperfusioninjurywithdiazoxideprotectstheblood-brainbarrier，whichmayduetotheincreasedexpressionoftightjunction-associatedproteinsClaudin-5andZo-1．Keywordsblood-brainbarrier

7、；diazoxide；ischemia／reperfusioninjury；tightjunctionClaudin·-5；Zo·-14·英文缩略语·5·论文·二氮嗪预处理对大鼠脑缺血再灌注后血脑屏障损伤的保护作用研究吉钉吉莉青2001年7月美国国立神经病学与卒中研究所(NationalInstitutesofNeurologicalDisordersandStroke，NINDS)提出了“神经血管单元”(neurovascularunit，NVU)的概念。近期研究发现卒中可以看做是多种细胞共同参与的脑功能紊乱。NVU构成了在卒中及其治疗中细胞一细胞

8、间传递信号和细胞一基质问相互影响的架构，为卒中研究提供了的新方向和药物干预的新靶点，其结构基础主要包括神经元