白灵菇优良杂交菌株的初步筛选

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1、摘要摘要本试验收集了生产上栽培的5个白灵菇菌株，通过拮抗试验、酯酶同工酶分析及出菇试验，对5个菌株进行了生理生化特性和栽培特性分析。在亲缘关系确定的基础上，选取生产性状优劣互补的“中农1号”和“中农翅鲍”两个菌株作为亲本。出菇收集担孢子，采用单孢分离技术分离两个菌株的担孢子，得到“中农1号”单核体54个，“中农翅鲍”单核体41个。选取生长较快的两亲本单核菌丝进行了杂交配对，得到150个杂交组合菌株，根据锁状联合有无进一步确定融合子。采用拮抗、酯酶同工酶、原基诱导及出菇等方法对融合子进行了进一步鉴定和评价分析，结果表明：（1）通过拮抗、酯酶同工酶和出菇验证，5个白灵菇分

2、为两大类群，一类以“中农1号”为代表菌株；一类以“中农翅鲍”为代表菌株。（2）通过镜检、拮抗、酯酶同工酶、原基诱导及出菇验证，150对杂交组合菌株中，99个菌株生长较快，有锁状联合，75个与两亲本有明显拮抗线，且具有两亲本菌株的典型酯酶同工酶条带，确定是杂交菌株，其中50个杂交子具有出菇能力，出菇验证初步得到了3个优良融合菌株。关键词：白灵菇，单孢分离，杂交育种，筛选I万方数据AbstractAbstractThephysiological,biochemicalandculturalcharacteristicsof5Pleurotuseryngiivar.tuol

3、iensisstrainswereanalysedbyantagonistictest,esteraseisozymeanalysisandfruitingtest.Using“zhongnong1”and“zhongnongchibao”withcomplementaryadvantageswereascrossingparentsonthefoundationofconfirminggeneticrelations.54monokaryonsfrom“zhongnong1”and41monokaryonsfrom“zhongnongchibao”wereobtai

4、nedbysinglesporeseparationtechnology.Twoabnormalsinglesporeisolateswerehybridizedandgot150hybridstrainaccordingtheexistenceofclamp-connection.Thefusantswereidentifiedbyantagonism,esteraseisozyme,primordiuminduction,fruitingtestandothermethods,theresultsshowedthat:(1)5Pleurotuseryngiivar

5、.tuoliensisweredividedintotwogreatcategoriesbyantagonism,esteraseisozymeandproducingmushroomdemonstration,onewastaken“zhongnong1”astherepresentativestrain;theotherwastaken“zhongnongchibao”astherepresentativestrain.(2)Among150hybridcombinationstrains,99strainsgrowedfasterwerewithclam-con

6、nection,75strainshadobviousantagonisticstreakswithtwoparents.equipwiththetypicalesyeraseisozymebeltoftwoparentstrainsbymicroscopicexamination,antagonism,esteraseisozymeandfruitingtestdemonstration.Theywereconfirmedashybridstrains,ofwhich50hybridscouldfruit.Thefruitingtestshowedthat3stra

7、inswereexcellentfusionhybrid.KeyWords:Pleurotuseryngiivar.tuoliensis,singlesporeseparation,hybridbreeding,screeningII万方数据目录目录摘要..............................................................IAbstract..........................................................Ⅱ第一章绪论................